Literature DB >> 20972747

Histopathology procedures: from tissue sampling to histopathological evaluation.

Mohamed Slaoui1, Laurence Fiette.   

Abstract

Histological procedures aim to provide good quality sections that can be used for a light microscopic evaluation of human or animal tissue changes in either spontaneous or induced diseases. Routinely, tissues are fixed with neutral formalin 10%, embedded in paraffin, and then manually sectioned with a microtome to obtain 4-5 μm-thick paraffin sections. Dewaxed sections are then stained with hematoxylin and eosin (H&E) or can be used for other purposes (special stains, immunohistochemistry, in situ hybridization, etc.). During this process, many steps and procedures are critical to ensure standard and interpretable sections. Key recomendations are given here to achieve this objective.

Entities:  

Mesh:

Year:  2011        PMID: 20972747     DOI: 10.1007/978-1-60761-849-2_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN: 1064-3745


  60 in total

1.  Detection and molecular characterization of kobuvirus from diarrheic goats in Minnesota.

Authors:  Nader M Sobhy; Aníbal G Armién; Arno Wünschmann; Dean Muldoon; Sagar M Goyal; Sunil K Mor
Journal:  J Vet Diagn Invest       Date:  2020-08-17       Impact factor: 1.279

2.  Electrospun emodin polyvinylpyrrolidone blended nanofibrous membrane: a novel medicated biomaterial for drug delivery and accelerated wound healing.

Authors:  Xin-Yi Dai; Wei Nie; Yong-Chun Wang; Yi Shen; Yan Li; Shu-Jie Gan
Journal:  J Mater Sci Mater Med       Date:  2012-08-09       Impact factor: 3.896

3.  Molecular identification of Mycobacterium spp. isolated from Brazilian wild boars.

Authors:  Bruna Correa Lopes; Anna Luiza Gisler Maciel; Márcia Regina Loiko; Thais Silveira Bueno; Mario Menezes Coppola; Angélica Cavalheiro Bertagnolli; Andreza Francisco Martins; Paulo Michel Roehe; David Driemeier; Fabiana Quoos Mayer
Journal:  Mol Biol Rep       Date:  2021-01-04       Impact factor: 2.316

4.  Protective effect of carnosine and N-acetylcysteine against sodium nitrite-induced oxidative stress and DNA damage in rat intestine.

Authors:  Fariheen Aisha Ansari; Aijaz Ahmed Khan; Riaz Mahmood
Journal:  Environ Sci Pollut Res Int       Date:  2018-05-04       Impact factor: 4.223

5.  Cyclooxygenase-2 inhibition for the prevention of subglottic stenosis.

Authors:  Selma Cetin; Allison B Tobey; Vlad C Sandulache; Tianbing Yang; Mark Barsic; Yan Lin; Joseph E Dohar; Patricia A Hebda
Journal:  Arch Otolaryngol Head Neck Surg       Date:  2012-10

6.  Ultrastructure and innervation of thumb carpometacarpal ligaments in surgical patients with osteoarthritis.

Authors:  Nathalie Mobargha; Cassie Ludwig; Amy L Ladd; Elisabet Hagert
Journal:  Clin Orthop Relat Res       Date:  2014-04       Impact factor: 4.176

7.  A Murine Orthotopic Allograft to Model Prostate Cancer Growth and Metastasis.

Authors:  Robert M Hughes; Brian W Simons; Paula J Hurley
Journal:  Bio Protoc       Date:  2017-02-20

8.  Generation of insulin-producing pancreatic β cells from multiple human stem cell lines.

Authors:  Nathaniel J Hogrebe; Kristina G Maxwell; Punn Augsornworawat; Jeffrey R Millman
Journal:  Nat Protoc       Date:  2021-08-04       Impact factor: 13.491

9.  Exopolysaccharides from Lactobacillus acidophilus modulates the antioxidant status of 1,2-dimethyl hydrazine-induced colon cancer rat model.

Authors:  Venkataraman Deepak; William Arputha Sundar; Sureshbabu Ram Kumar Pandian; Shiva D Sivasubramaniam; Nellaiah Hariharan; Krishnan Sundar
Journal:  3 Biotech       Date:  2021-04-19       Impact factor: 2.406

10.  Analysis pipelines for cancer genome sequencing in mice.

Authors:  Sebastian Lange; Thomas Engleitner; Sebastian Mueller; Roman Maresch; Maximilian Zwiebel; Laura González-Silva; Günter Schneider; Ruby Banerjee; Fengtang Yang; George S Vassiliou; Mathias J Friedrich; Dieter Saur; Ignacio Varela; Roland Rad
Journal:  Nat Protoc       Date:  2020-01-06       Impact factor: 13.491

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.