PURPOSE: To define germinal vesicles (GV) by morphometric and morphologic examination and by chromatin compaction and to assess their spontaneous nuclear and cytoplasmic competence. MATERIALS: 131 GV were cultured for 42.7 ± 2.4 h. Nuclear maturation was evaluated at four time points. Sixty-seven in vitro and twenty-five in vivo metaphase II (MII) were activated. Parthenotes with 2 PB and one pronucleus (NA) were studied for ploidy. RESULTS: A total of 74.8% GV matured to MII: 55% at 21.4 ± 2.4 h and 47.3% in the following 24 h. Artificial activation induced NA in 79.2% of in vivo-MII and in 22.4% of in vitro-MII. All NA were haploid. CONCLUSIONS: GV spontaneously mature at the nuclear level. Their NA are haploid, but their cytoplasmic competence is compromised. Variables were not found to be predictors of oocyte competence, probably due to our population being homogeneous with respect to most of the variables studied.
PURPOSE: To define germinal vesicles (GV) by morphometric and morphologic examination and by chromatin compaction and to assess their spontaneous nuclear and cytoplasmic competence. MATERIALS: 131 GV were cultured for 42.7 ± 2.4 h. Nuclear maturation was evaluated at four time points. Sixty-seven in vitro and twenty-five in vivo metaphase II (MII) were activated. Parthenotes with 2 PB and one pronucleus (NA) were studied for ploidy. RESULTS: A total of 74.8% GV matured to MII: 55% at 21.4 ± 2.4 h and 47.3% in the following 24 h. Artificial activation induced NA in 79.2% of in vivo-MII and in 22.4% of in vitro-MII. All NA were haploid. CONCLUSIONS:GV spontaneously mature at the nuclear level. Their NA are haploid, but their cytoplasmic competence is compromised. Variables were not found to be predictors of oocyte competence, probably due to our population being homogeneous with respect to most of the variables studied.
Authors: Christian S Ottolini; Antonio Capalbo; Louise Newnham; Danilo Cimadomo; Senthilkumar A Natesan; Eva R Hoffmann; Filippo M Ubaldi; Laura Rienzi; Alan H Handyside Journal: Nat Protoc Date: 2016-06-16 Impact factor: 13.491
Authors: Jennifer R Gruhn; Agata P Zielinska; Vallari Shukla; Robert Blanshard; Antonio Capalbo; Danilo Cimadomo; Dmitry Nikiforov; Andrew Chi-Ho Chan; Louise J Newnham; Ivan Vogel; Catello Scarica; Marta Krapchev; Deborah Taylor; Stine Gry Kristensen; Junping Cheng; Erik Ernst; Anne-Mette Bay Bjørn; Lotte Berdiin Colmorn; Martyn Blayney; Kay Elder; Joanna Liss; Geraldine Hartshorne; Marie Louise Grøndahl; Laura Rienzi; Filippo Ubaldi; Rajiv McCoy; Krzysztof Lukaszuk; Claus Yding Andersen; Melina Schuh; Eva R Hoffmann Journal: Science Date: 2019-09-27 Impact factor: 47.728