OBJECTIVE: The purpose of this study was to analyze DNA methylation profiles among different types of ovarian serous neoplasm, which is a task that has not been performed. STUDY DESIGN: The Illumina beads array (Illumina Inc, San Diego, CA) was used to profile DNA methylation in enriched tumor cells that had been isolated from 75 benign and malignant serous tumor tissues and 6 tumor-associated stromal cell cultures. RESULTS: We found significantly fewer hypermethylated genes in high-grade serous carcinomas than in low-grade serous carcinoma and borderline tumors, which in turn had fewer hypermethylated genes than serous cystadenoma. Unsupervised analysis identified that serous cystadenoma, serous borderline tumor, and low-grade serous carcinomas tightly clustered together and were clearly different from high-grade serous carcinomas. We also performed supervised analysis to identify differentially methylated genes that may contribute to group separation. CONCLUSION: The findings support the view that low-grade and high-grade serous carcinomas are distinctly different with low-grade, but not high-grade, serous carcinomas that are related to serous borderline tumor and cystadenoma.
OBJECTIVE: The purpose of this study was to analyze DNA methylation profiles among different types of ovarian serous neoplasm, which is a task that has not been performed. STUDY DESIGN: The Illumina beads array (Illumina Inc, San Diego, CA) was used to profile DNA methylation in enriched tumor cells that had been isolated from 75 benign and malignant serous tumor tissues and 6 tumor-associated stromal cell cultures. RESULTS: We found significantly fewer hypermethylated genes in high-grade serous carcinomas than in low-grade serous carcinoma and borderline tumors, which in turn had fewer hypermethylated genes than serous cystadenoma. Unsupervised analysis identified that serous cystadenoma, serous borderline tumor, and low-grade serous carcinomas tightly clustered together and were clearly different from high-grade serous carcinomas. We also performed supervised analysis to identify differentially methylated genes that may contribute to group separation. CONCLUSION: The findings support the view that low-grade and high-grade serous carcinomas are distinctly different with low-grade, but not high-grade, serous carcinomas that are related to serous borderline tumor and cystadenoma.
Authors: Inmaculada Ibanez de Caceres; Cristina Battagli; Manel Esteller; James G Herman; Essel Dulaimi; Mitchell I Edelson; Cynthia Bergman; Hormoz Ehya; Burton L Eisenberg; Paul Cairns Journal: Cancer Res Date: 2004-09-15 Impact factor: 12.701
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Authors: Erin R King; Zhifei Zu; Yvonne T M Tsang; Michael T Deavers; Anais Malpica; Samuel C Mok; David M Gershenson; Kwong-Kwok Wong Journal: Gynecol Oncol Date: 2011-07-02 Impact factor: 5.482
Authors: Felix Zeppernick; Laura Ardighieri; Charlotte G Hannibal; Russell Vang; Jette Junge; Susanne K Kjaer; Rugang Zhang; Robert J Kurman; Ie-Ming Shih Journal: Am J Surg Pathol Date: 2014-12 Impact factor: 6.394
Authors: Verena Kirn; Rong Shi; Sabine Heublein; Julia Knabl; Margit Guenthner-Biller; Ulrich Andergassen; Claudius Fridrich; Wolfram Malter; Jan Harder; Klaus Friese; Doris Mayr; Udo Jeschke Journal: J Cancer Res Clin Oncol Date: 2014-06-08 Impact factor: 4.553
Authors: Yvonne T Tsang; Michael T Deavers; Charlotte C Sun; Suet-Yan Kwan; Eric Kuo; Anais Malpica; Samuel C Mok; David M Gershenson; Kwong-Kwok Wong Journal: J Pathol Date: 2013-12 Impact factor: 7.996