Literature DB >> 20962143

False-negative PCR result due to gene polymorphism: the example of Neisseria meningitidis.

Katia Jaton1, Béatrice Ninet, Jacques Bille, Gilbert Greub.   

Abstract

Early treatment of meningococcal meningitis is mandatory but may negate the cerebrospinal fluid culture. Etiological diagnosis then mainly relies on PCR. Here, we report a case of false-negative results for real-time PCR for a Neisseria meningitidis serogroup B isolate with a polymorphism in the ctrA gene.

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Year:  2010        PMID： 20962143      PMCID： PMC3008480          DOI： 10.1128/JCM.01766-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  17 in total

1.  Multiple nucleotide substitutions in the Neisseria meningitidis serogroup C ctrA gene cause false-negative detection by real-time PCR.

Authors:  Francesca Cavrini; Giovanna Liguori; Arianna Andreoli; Vittorio Sambri
Journal:  J Clin Microbiol       Date:  2010-06-02       Impact factor: 5.948

2.  Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae.

Authors:  G Tzanakaki; M Tsopanomichalou; K Kesanopoulos; R Matzourani; M Sioumala; A Tabaki; J Kremastinou
Journal:  Clin Microbiol Infect       Date:  2005-05       Impact factor: 8.067

Review 3.  Nucleic Acid amplification tests for diagnosis of Bordetella infections.

Authors:  M Riffelmann; C H Wirsing von König; V Caro; N Guiso
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

Review 4.  Serotype antigens of Neisseria meningitidis and a proposed scheme for designation of serotypes.

Authors:  C E Frasch; W D Zollinger; J T Poolman
Journal:  Rev Infect Dis       Date:  1985 Jul-Aug

5.  Genetic relationships between clinical isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: characterization of "Atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes.

Authors:  A M Whatmore; A Efstratiou; A P Pickerill; K Broughton; G Woodard; D Sturgeon; R George; C G Dowson
Journal:  Infect Immun       Date:  2000-03       Impact factor: 3.441

6.  Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing.

Authors:  D Goldenberger; A Künzli; P Vogt; R Zbinden; M Altwegg
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

7.  Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis.

Authors:  Muhamed-Kheir Taha; Jean-Michel Alonso; Mary Cafferkey; Dominique A Caugant; Stuart C Clarke; Mathew A Diggle; Andrew Fox; Matthias Frosch; Stephen J Gray; Malcolm Guiver; Sigrid Heuberger; Jitka Kalmusova; Konstantinos Kesanopoulos; Anne-Marie Klem; Paula Kriz; John Marsh; Paula Mölling; Karen Murphy; Per Olcén; Oumar Sanou; Georgina Tzanakaki; Ulrich Vogel
Journal:  J Clin Microbiol       Date:  2005-01       Impact factor: 5.948

Review 8.  Multilocus sequence typing for global surveillance of meningococcal disease.

Authors:  Carina Brehony; Keith A Jolley; Martin C J Maiden
Journal:  FEMS Microbiol Rev       Date:  2006-12-01       Impact factor: 16.408

9.  Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of meningitis and septicemia using real-time PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; A J Fox; E B Kaczmarski
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

10.  Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

Authors:  Johanna Jefferies; Leena Nieminen; Lea-Ann Kirkham; Calum Johnston; Andrew Smith; Tim J Mitchell
Journal:  J Bacteriol       Date:  2006-10-27       Impact factor: 3.490
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  6 in total

1.  The Impact of Fast Microbiology in Intensive Care Units in the Era of Antibiotic Resistance: An Observational Retrospective Study.

Authors:  Mirko Muzzi; Bruno Viaggi; Sergio Fabbri; Lorenzo Bechi; Caterina Scirè-Calabrisotto; Gianluca Villa; Stefano Romagnoli
Journal:  Curr Microbiol       Date:  2022-01-31       Impact factor: 2.188

2.  Limitation of ctrA as a Target for Neisseria meningitidis Identification and Potential Alternative Targets.

Authors:  Imogen Sirluck-Schroeder; Ghada N Al-Rawahi; Vijay Gadkar; Linda Hoang; Raymond Tsang; Peter Tilley
Journal:  J Clin Microbiol       Date:  2022-05-23       Impact factor: 11.677

3.  Optimization of Molecular Approaches to Genogroup Neisseria meningitidis Carriage Isolates and Implications for Monitoring the Impact of New Serogroup B Vaccines.

Authors:  Eduardo Rojas; Johanna Hoyos; Neil J Oldfield; Philip Lee; Mike Flint; C Hal Jones; Dlawer A A Ala'Aldeen; Kathrin U Jansen; Annaliesa S Anderson
Journal:  PLoS One       Date:  2015-07-06       Impact factor: 3.240

4.  Distinct Genomic Features Characterize Two Clades of Corynebacterium diphtheriae: Proposal of Corynebacterium diphtheriae Subsp. diphtheriae Subsp. nov. and Corynebacterium diphtheriae Subsp. lausannense Subsp. nov.

Authors:  Florian Tagini; Trestan Pillonel; Antony Croxatto; Claire Bertelli; Angela Koutsokera; Alban Lovis; Gilbert Greub
Journal:  Front Microbiol       Date:  2018-08-17       Impact factor: 5.640

Review 5.  Towards multitarget testing in molecular microbiology.

Authors:  Deborah Steensels; Anne Vankeerberghen; Hans De Beenhouwer
Journal:  Int J Microbiol       Date:  2013-11-25

6.  Comparison of PCR-based methods for the simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in clinical samples.

Authors:  Ivano de Filippis; Claudia Ferreira de Andrade; Nathalia Caldeira; Aline Carvalho de Azevedo; Antonio Eugenio de Almeida
Journal:  Braz J Infect Dis       Date:  2016-05-30       Impact factor: 3.257
  6 in total

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