Literature DB >> 17071760

Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

Johanna Jefferies1, Leena Nieminen, Lea-Ann Kirkham, Calum Johnston, Andrew Smith, Tim J Mitchell.   

Abstract

We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mitilysin extracellularly. Certain alleles of mitilysin are not recognized by a monoclonal antibody raised to the related toxin pneumolysin. Based on enzyme-linked immunosorbent assay and neutralization assay results, one isolate of S. mitis may produce a further hemolytic toxin in addition to mitilysin. As genetic exchange is known to occur between S. mitis and Streptococcus pneumoniae, this finding may have implications for the development of vaccines or therapies for pneumococcal disease that are based on pneumolysin.

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Year:  2006        PMID： 17071760      PMCID： PMC1797409          DOI： 10.1128/JB.01092-06

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


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