Literature DB >> 20957255

Increasing the stability of an enzyme toward hostile organic solvents by directed evolution based on iterative saturation mutagenesis using the B-FIT method.

Manfred T Reetz1, Pankaj Soni, Layla Fernández, Yosephine Gumulya, José D Carballeira.   

Abstract

Mutants of the lipase from Bacillus subtilis, previously engineered for enhanced thermostability using directed evolution based on the B-FIT method, show significantly increased tolerance to hostile organic solvents.

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Year:  2010        PMID： 20957255     DOI： 10.1039/c0cc02657c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  29 in total

1.  Directed evolution of an ultrastable carbonic anhydrase for highly efficient carbon capture from flue gas.

Authors:  Oscar Alvizo; Luan J Nguyen; Christopher K Savile; Jamie A Bresson; Satish L Lakhapatri; Earl O P Solis; Richard J Fox; James M Broering; Michael R Benoit; Sabrina A Zimmerman; Scott J Novick; Jack Liang; James J Lalonde
Journal:  Proc Natl Acad Sci U S A       Date:  2014-11-03       Impact factor: 11.205

2.  Directed evolution methods for overcoming trade-offs between protein activity and stability.

Authors:  Samuel D Stimple; Matthew D Smith; Peter M Tessier
Journal:  AIChE J       Date:  2019-10-09       Impact factor: 3.993

Review 3.  Biocatalyst development by directed evolution.

Authors:  Meng Wang; Tong Si; Huimin Zhao
Journal:  Bioresour Technol       Date:  2012-01-21       Impact factor: 9.642

4.  Protein engineering by random mutagenesis and structure-guided consensus of Geobacillus stearothermophilus Lipase T6 for enhanced stability in methanol.

Authors:  Adi Dror; Einav Shemesh; Natali Dayan; Ayelet Fishman
Journal:  Appl Environ Microbiol       Date:  2013-12-20       Impact factor: 4.792

Review 5.  Adaptational properties and applications of cold-active lipases from psychrophilic bacteria.

Authors:  Jonathan Maiangwa; Mohd Shukuri Mohamad Ali; Abu Bakar Salleh; Raja Noor Zaliha Raja Abd Rahman; Fairolniza Mohd Shariff; Thean Chor Leow
Journal:  Extremophiles       Date:  2014-12-04       Impact factor: 2.395

6.  Characterization and evolution of a metagenome-derived lipase towards enhanced enzyme activity and thermostability.

Authors:  Rakesh Kumar; Monika Sharma; Ranvir Singh; Jagdeep Kaur
Journal:  Mol Cell Biochem       Date:  2012-10-27       Impact factor: 3.396

7.  Enhancing subtilisin thermostability through a modified normalized B-factor analysis and loop-grafting strategy.

Authors:  Heng Tang; Ke Shi; Cheng Shi; Hideki Aihara; Juan Zhang; Guocheng Du
Journal:  J Biol Chem       Date:  2019-10-15       Impact factor: 5.157

Review 8.  Cold Active Lipases: Biocatalytic Tools for Greener Technology.

Authors:  Nutan Mhetras; Vidhyashri Mapare; Digambar Gokhale
Journal:  Appl Biochem Biotechnol       Date:  2021-02-05       Impact factor: 2.926

9.  Calcium-ion-induced stabilization of the protease from Bacillus cereus WQ9-2 in aqueous hydrophilic solvents: effect of calcium ion binding on the hydration shell and intramolecular interactions.

Authors:  Jiaxing Xu; Yu Zhuang; Bin Wu; Long Su; Bingfang He
Journal:  J Biol Inorg Chem       Date:  2013-01-16       Impact factor: 3.358

10.  Library design and screening protocol for artificial metalloenzymes based on the biotin-streptavidin technology.

Authors:  Hendrik Mallin; Martina Hestericová; Raphael Reuter; Thomas R Ward
Journal:  Nat Protoc       Date:  2016-03-31       Impact factor: 13.491
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