BACKGROUND & AIMS: Hepatocellular carcinoma (HCC) is associated with a high potential for metastasis and disease recurrence, even after surgical resection. The cancer stem cell (CSC) hypothesis proposes that CSCs are responsible for chemo-resistance, recurrence, and metastasis. Dysadherin is a prognostic indicator of metastasis and poor survival in many different cancer types. In this study, we investigated the possible link between dysadherin and CSC in HCC. METHODS: We analyzed the functional implications of dysadherin on cancer stemness by modification of the dysadherin gene in HCC cell lines. RESULTS: The transfection of dysadherin cDNA into the liver cancer cell line PLC/PRF/5 enhanced the properties of CSCs, including anti-apoptosis, their sphere-forming ability, side population phenotype, and tumor initiation ability in vivo. Furthermore, knockdown of dysadherin in the liver cancer cell line SK-Hep1 suppressed its stem cell-like properties. CONCLUSIONS: These results show that dysadherin give rise to properties of CSC in HCC. Therefore, these findings suggest that dysadherin may be a potential molecular prognostic marker of HCC and may aid in the development of more effective therapies.
BACKGROUND & AIMS:Hepatocellular carcinoma (HCC) is associated with a high potential for metastasis and disease recurrence, even after surgical resection. The cancer stem cell (CSC) hypothesis proposes that CSCs are responsible for chemo-resistance, recurrence, and metastasis. Dysadherin is a prognostic indicator of metastasis and poor survival in many different cancer types. In this study, we investigated the possible link between dysadherin and CSC in HCC. METHODS: We analyzed the functional implications of dysadherin on cancer stemness by modification of the dysadherin gene in HCC cell lines. RESULTS: The transfection of dysadherin cDNA into the liver cancer cell line PLC/PRF/5 enhanced the properties of CSCs, including anti-apoptosis, their sphere-forming ability, side population phenotype, and tumor initiation ability in vivo. Furthermore, knockdown of dysadherin in the liver cancer cell line SK-Hep1 suppressed its stem cell-like properties. CONCLUSIONS: These results show that dysadherin give rise to properties of CSC in HCC. Therefore, these findings suggest that dysadherin may be a potential molecular prognostic marker of HCC and may aid in the development of more effective therapies.
Authors: Catharina Steentoft; Max Fuhrmann; Federico Battisti; Julie Van Coillie; Thomas D Madsen; Diana Campos; Adnan Halim; Sergey Y Vakhrushev; Hiren J Joshi; Hans Schreiber; Ulla Mandel; Yoshiki Narimatsu Journal: Glycobiology Date: 2019-04-01 Impact factor: 4.313
Authors: Elmira Tokhtaeva; Haying Sun; Nimrod Deiss-Yehiely; Yi Wen; Pritin N Soni; Nieves M Gabrielli; Elizabeth A Marcus; Karen M Ridge; George Sachs; Mónica Vazquez-Levin; Jacob I Sznajder; Olga Vagin; Laura A Dada Journal: J Cell Sci Date: 2016-05-03 Impact factor: 5.285
Authors: Jonathan Noujaim; Khin Thway; Zia Bajwa; Ayeza Bajwa; Robert G Maki; Robin L Jones; Charles Keller Journal: Front Oncol Date: 2015-08-17 Impact factor: 6.244
Authors: K Juurikka; A Dufour; K Pehkonen; B Mainoli; P Campioni Rodrigues; N Solis; T Klein; P Nyberg; C M Overall; T Salo; P Åström Journal: Oncogenesis Date: 2021-05-31 Impact factor: 7.485