PURPOSE: In this study, we investigated the effect of platelet-rich plasma (PRP) and bone marrow-derived stromal cell (BMSC)-seeded interposition in an in vitro canine tendon repair model. METHODS: Bone marrow, peripheral blood, and tendons were harvested from mixed-breed dogs. Bone marrow-derived stromal cells were cultured and passaged from adherent cells of bone marrow suspension. Platelet-rich plasma was purified from peripheral blood using a commercial kit. A total of 192 flexor digitorum profundus tendons were used for the study. Tendons repaired with a simple suture were used as a control group. In treatment groups, a collagen gel patch was interposed at the tendon repair site before suture. There were 3 treatment groups, according to the type of collagen patch: a patch with PRP, a patch with BMSC, and a patch with PRP and BMSC. The repaired tendons were evaluated by biomechanical testing and by histological survey after 2 and 4 weeks in tissue culture. To evaluate viability, cells were labeled with PKH26 red fluorescent cell linker (Sigma, St. Louis, MO) and surveyed under confocal microscopy after culture. RESULTS: The maximum breaking strength and stiffness of the healing tendons with the BMSC-seeded PRP patch were significantly higher than those of the healing tendons without a patch or with a cell-seeded patch (p < .02). Viable BMSCs were present at both 2 and 4 weeks. CONCLUSIONS: Platelet-rich plasma enhanced the effect of BMSC-seeded collagen gel interposition in this in vitro model. Based on these results, we now plan to investigate this effect in vivo.
PURPOSE: In this study, we investigated the effect of platelet-rich plasma (PRP) and bone marrow-derived stromal cell (BMSC)-seeded interposition in an in vitro canine tendon repair model. METHODS: Bone marrow, peripheral blood, and tendons were harvested from mixed-breed dogs. Bone marrow-derived stromal cells were cultured and passaged from adherent cells of bone marrow suspension. Platelet-rich plasma was purified from peripheral blood using a commercial kit. A total of 192 flexor digitorum profundus tendons were used for the study. Tendons repaired with a simple suture were used as a control group. In treatment groups, a collagen gel patch was interposed at the tendon repair site before suture. There were 3 treatment groups, according to the type of collagen patch: a patch with PRP, a patch with BMSC, and a patch with PRP and BMSC. The repaired tendons were evaluated by biomechanical testing and by histological survey after 2 and 4 weeks in tissue culture. To evaluate viability, cells were labeled with PKH26 red fluorescent cell linker (Sigma, St. Louis, MO) and surveyed under confocal microscopy after culture. RESULTS: The maximum breaking strength and stiffness of the healing tendons with the BMSC-seeded PRP patch were significantly higher than those of the healing tendons without a patch or with a cell-seeded patch (p < .02). Viable BMSCs were present at both 2 and 4 weeks. CONCLUSIONS: Platelet-rich plasma enhanced the effect of BMSC-seeded collagen gel interposition in this in vitro model. Based on these results, we now plan to investigate this effect in vivo.
Authors: R E Marx; E R Carlson; R M Eichstaedt; S R Schimmele; J E Strauss; K R Georgeff Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Date: 1998-06
Authors: Kosuke Uehara; Chunfeng Zhao; Anne Gingery; Andrew R Thoreson; Kai-Nan An; Peter C Amadio Journal: J Bone Joint Surg Am Date: 2015-11-04 Impact factor: 5.284
Authors: Chunfeng Zhao; Yasuhiro Ozasa; Ramona L Reisdorf; Andrew R Thoreson; Gregory D Jay; Kai-Nan An; Peter C Amadio Journal: Clin Orthop Relat Res Date: 2014-06-07 Impact factor: 4.176
Authors: Yasuhiro Ozasa; Anne Gingery; Andrew R Thoreson; Kai-Nan An; Chunfeng Zhao; Peter C Amadio Journal: J Hand Surg Am Date: 2014-06-06 Impact factor: 2.230
Authors: Vaclav Prochazka; Hana Klosova; Jiri Stetinsky; Jaromir Gumulec; Katerina Vitkova; Dana Salounova; Jana Dvorackova; Hana Bielnikova; Petr Klement; Veronika Levakova; Tomas Ocelka; Lubomir Pavliska; Pavel Kovanic; Giannoula Lakka Klement Journal: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub Date: 2013-09-27 Impact factor: 1.245
Authors: Kosuke Uehara; Chunfeng Zhao; Anne Gingery; Andrew R Thoreson; Kai-Nan An; Peter C Amadio Journal: J Orthop Sci Date: 2020-08-16 Impact factor: 1.805
Authors: Francesca Salamanna; Francesca Veronesi; Melania Maglio; Elena Della Bella; Maria Sartori; Milena Fini Journal: Biomed Res Int Date: 2015-05-05 Impact factor: 3.411