Literature DB >> 20949431

Purification of arsenic (+3 oxidation state) methyltransferase from rat liver cytosol.

Zuzana Drobna1, Miroslav Styblo, David J Thomas.   

Abstract

Demonstrating the enzymatic basis of arsenic methylation is critical to further studies of the pathway for the conversion of inorganic arsenic into a variety of methylated metabolites. This protocol describes a procedure for the purification of an arsenic methyltransferase from rat liver cytosol. Purification of this enzyme and subsequent cloning of its gene has permitted studies of enzyme structure and function, and has lead to the identification of orthologous genes in genomes of organisms ranging in complexity from sea urchins to humans. These proteins are referred to as arsenic (+3 oxidation state) methyltransferases.
© 2009 by John Wiley & Sons, Inc.

Entities:  

Mesh：

Substances：

Year:  2009        PMID： 20949431      PMCID： PMC4500041          DOI： 10.1002/0471140856.tx0434s42

Source DB:  PubMed          Journal:  Curr Protoc Toxicol        ISSN： 1934-9254


  10 in total

Review 1.  Arsenic (+3 oxidation state) methyltransferase and the methylation of arsenicals.

Authors:  David J Thomas; Jiaxin Li; Stephen B Waters; Weibing Xing; Blakely M Adair; Zuzana Drobna; Vicenta Devesa; Miroslav Styblo
Journal:  Exp Biol Med (Maywood)       Date:  2007-01

Review 2.  Why purify enzymes?

Authors:  A Kornberg
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

3.  Widespread occurrence of three sequence motifs in diverse S-adenosylmethionine-dependent methyltransferases suggests a common structure for these enzymes.

Authors:  R M Kagan; S Clarke
Journal:  Arch Biochem Biophys       Date:  1994-05-01       Impact factor: 4.013

4.  A novel S-adenosyl-L-methionine:arsenic(III) methyltransferase from rat liver cytosol.

Authors:  Shan Lin; Qing Shi; F Brent Nix; Miroslav Styblo; Melinda A Beck; Karen M Herbin-Davis; Larry L Hall; Josef B Simeonsson; David J Thomas
Journal:  J Biol Chem       Date:  2002-01-14       Impact factor: 5.157

5.  Enzymatic methylation of arsenic compounds. III. The marmoset and tamarin, but not the rhesus, monkeys are deficient in methyltransferases that methylate inorganic arsenic.

Authors:  R A Zakharyan; E Wildfang; H V Aposhian
Journal:  Toxicol Appl Pharmacol       Date:  1996-09       Impact factor: 4.219

6.  High-throughput identification of catalytic redox-active cysteine residues.

Authors:  Dmitri E Fomenko; Weibing Xing; Blakely M Adair; David J Thomas; Vadim N Gladyshev
Journal:  Science       Date:  2007-01-19       Impact factor: 47.728

7.  Purification, characterization, and cloning of an S-adenosylmethionine-dependent 3-amino-3-carboxypropyltransferase in nocardicin biosynthesis.

Authors:  A M Reeve; S D Breazeale; C A Townsend
Journal:  J Biol Chem       Date:  1998-11-13       Impact factor: 5.157

8.  Enzymatic methylation of arsenic compounds. VI. Characterization of hamster liver arsenite and methylarsonic acid methyltransferase activities in vitro.

Authors:  E Wildfang; R A Zakharyan; H V Aposhian
Journal:  Toxicol Appl Pharmacol       Date:  1998-10       Impact factor: 4.219

9.  Enzymatic methylation of arsenic compounds: assay, partial purification, and properties of arsenite methyltransferase and monomethylarsonic acid methyltransferase of rabbit liver.

Authors:  R Zakharyan; Y Wu; G M Bogdan; H V Aposhian
Journal:  Chem Res Toxicol       Date:  1995-12       Impact factor: 3.739

Review 10.  Human and orthologous gene nomenclature.

Authors:  Mathew W Wright; Elspeth A Bruford
Journal:  Gene       Date:  2006-01-23       Impact factor: 3.688
  10 in total
  1 in total

Review 1.  Arsenic, asbestos and radon: emerging players in lung tumorigenesis.

Authors:  Roland Hubaux; Daiana D Becker-Santos; Katey S S Enfield; Stephen Lam; Wan L Lam; Victor D Martinez
Journal:  Environ Health       Date:  2012-11-22       Impact factor: 5.984
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.