Literature DB >> 20943869

Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus.

Surbhi Malhotra-Kumar1, Liesbet Van Heirstraeten, Andie Lee, José Cortinas Abrahantes, Christine Lammens, Evelyn Vanhommerig, Geert Molenberghs, Marc Aerts, Stephan Harbarth, Herman Goossens.   

Abstract

The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20943869      PMCID： PMC3008470          DOI： 10.1128/JCM.00004-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

1.  Methicillin-resistant Staphylococcus aureus whole-body decolonization among hospitalized patients with variable site colonization by using mupirocin in combination with octenidine dihydrochloride.

Authors:  U Rohr; C Mueller; M Wilhelm; G Muhr; S Gatermann
Journal:  J Hosp Infect       Date:  2003-08       Impact factor: 3.926

2.  Novel non-mecA-containing staphylococcal chromosomal cassette composite island containing pbp4 and tagF genes in a commensal staphylococcal species: a possible reservoir for antibiotic resistance islands in Staphylococcus aureus.

Authors:  Kanokporn Mongkolrattanothai; Susan Boyle; Trudy V Murphy; Robert S Daum
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

3.  Evaluation of the Xpert MRSA assay for rapid detection of methicillin-resistant Staphylococcus aureus from nares swabs of geriatric hospitalized patients and failure to detect a specific SCCmec type IV variant.

Authors:  C Laurent; P Bogaerts; D Schoevaerdts; O Denis; A Deplano; C Swine; M J Struelens; Y Glupczynski
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2010-05-29       Impact factor: 3.267

4.  Failure of the BD GeneOhm StaphS/R assay for identification of Australian methicillin-resistant Staphylococcus aureus strains: duplex assays as the "gold standard" in settings of unknown SCCmec epidemiology.

Authors:  Lee Thomas; Sebastiaan van Hal; Matthew O'Sullivan; Pierre Kyme; Jon Iredell
Journal:  J Clin Microbiol       Date:  2008-10-22       Impact factor: 5.948

5.  Single-nucleotide polymorphism in the SCCmec-orfX junction distinguishes between livestock-associated MRSA CC398 and human epidemic MRSA strains.

Authors:  U Reischl; J Frick; S Hoermansdorfer; H Melzl; M Bollwein; H J Linde; K Becker; R Köck; C Tuschak; U Busch; A Sing
Journal:  Euro Surveill       Date:  2009-12-10

6.  Impact of a short period of pre-enrichment on detection and bacterial loads of methicillin-resistant Staphylococcus aureus from screening specimens.

Authors:  L Van Heirstraeten; J Cortiñas Abrahantes; C Lammens; A Lee; S Harbarth; G Molenberghs; M Aerts; H Goossens; S Malhotra-Kumar
Journal:  J Clin Microbiol       Date:  2009-08-12       Impact factor: 5.948

7.  Comparison of MRSASelect Agar, CHROMagar Methicillin-Resistant Staphylococcus aureus (MRSA) Medium, and Xpert MRSA PCR for detection of MRSA in Nares: diagnostic accuracy for surveillance samples with various bacterial densities.

Authors:  D M Wolk; J L Marx; L Dominguez; D Driscoll; R B Schifman
Journal:  J Clin Microbiol       Date:  2009-10-14       Impact factor: 5.948

8.  Comparison of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay, BD GeneOhm MRSA assay, and culture for detection of nasal and cutaneous groin colonization by MRSA.

Authors:  P G Kelley; E A Grabsch; B P Howden; W Gao; M L Grayson
Journal:  J Clin Microbiol       Date:  2009-08-26       Impact factor: 5.948

9.  New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci.

Authors:  A Huletsky; R Giroux; V Rossbach; M Gagnon; M Vaillancourt; M Bernier; F Gagnon; K Truchon; M Bastien; F J Picard; A van Belkum; M Ouellette; P H Roy; M G Bergeron
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

10.  Evaluation of chromogenic media for detection of methicillin-resistant Staphylococcus aureus.

Authors:  Surbhi Malhotra-Kumar; José Cortinas Abrahantes; Wilber Sabiiti; Christine Lammens; Greetje Vercauteren; Margareta Ieven; Geert Molenberghs; Marc Aerts; Herman Goossens
Journal:  J Clin Microbiol       Date:  2010-02-17       Impact factor: 5.948
View more
  9 in total

Review 1.  Are we ready for novel detection methods to treat respiratory pathogens in hospital-acquired pneumonia?

Authors:  Andrea Endimiani; Kristine M Hujer; Andrea M Hujer; Sebastian Kurz; Michael R Jacobs; David S Perlin; Robert A Bonomo
Journal:  Clin Infect Dis       Date:  2011-05       Impact factor: 9.079

2.  Positive predictive value of the Xpert MRSA assay diagnostic for universal patient screening at hospital admission: influence of the local ecology.

Authors:  S Roisin; C Laurent; C Nonhoff; A Deplano; M Hallin; B Byl; M J Struelens; O Denis
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-08-28       Impact factor: 3.267

3.  Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay.

Authors:  Paul D Stamper; Lisa Louie; Henry Wong; Andrew E Simor; Jason E Farley; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2011-02-09       Impact factor: 5.948

4.  Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay.

Authors:  Kashonda Kelley; Angela Cosman; Phillip Belgrader; Brenda Chapman; Donna C Sullivan
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

5.  Searching Biomarkers in the Sequenced Genomes of Staphylococcus for their Rapid Identification.

Authors:  Ravi Kumar; Shikha Koul; Prasun Kumar; Vipin Chandra Kalia
Journal:  Indian J Microbiol       Date:  2016-01-20       Impact factor: 2.461

6.  Generation of Recombinant Antibodies in HEK293F Cells for the Detection of Staphylococcus aureus.

Authors:  Joo-Kyung Kim; Gyu-Min Lim; Eun-Jung Kim; Wooseong Kim; Chang-Soo Lee; Byung-Gee Kim; Hee-Jin Jeong
Journal:  ACS Omega       Date:  2022-03-09

7.  Clinical Relevance of Xpert MRSA/SA in Guiding Therapeutic Decisions for Staphylococcal Infections: A Diagnostic Test Accuracy Analysis.

Authors:  Suvash Chandra Ojha; Ke Chen; Changfeng Sun; Sarfraz Ahmed; Yun-Jian Sheng; Cun-Liang Deng
Journal:  Infect Dis Ther       Date:  2022-04-22

8.  Clinical utility of the Xpert MRSA assay for early detection of methicillin-resistant Staphylococcus aureus.

Authors:  Ae-Chin Oh; Jin Kyung Lee; Ha Na Lee; Young Jun Hong; Yoon Hwan Chang; Seok-Il Hong; Dong Ho Kim
Journal:  Mol Med Rep       Date:  2012-10-09       Impact factor: 2.952

9.  Interventions to reduce colonisation and transmission of antimicrobial-resistant bacteria in intensive care units: an interrupted time series study and cluster randomised trial.

Authors:  Lennie P G Derde; Ben S Cooper; Herman Goossens; Surbhi Malhotra-Kumar; Rob J L Willems; Marek Gniadkowski; Waleria Hryniewicz; Joanna Empel; Mirjam J D Dautzenberg; Djillali Annane; Irene Aragão; Annie Chalfine; Uga Dumpis; Francisco Esteves; Helen Giamarellou; Igor Muzlovic; Giuseppe Nardi; George L Petrikkos; Viktorija Tomic; Antonio Torres Martí; Pascal Stammet; Christian Brun-Buisson; Marc J M Bonten
Journal:  Lancet Infect Dis       Date:  2013-10-23       Impact factor: 25.071
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.