Literature DB >> 20943867

Novel multiplex PCR using dual-priming oligonucleotides for detection and discrimination of the Mycobacterium tuberculosis complex and M. bovis BCG.

Hye Ryun Lee1, So Yeon Kim, Ho Eun Chang, Sang Hoon Song, Hye Seung Lee, Kyoung Un Park, Junghan Song, Eui-Chong Kim.   

Abstract

We developed a novel multiplex PCR assay using dual-priming oligonucleotide primers targeting the RD1 gene for simultaneous identification of the Mycobacterium tuberculosis complex and M. bovis bacillus Calmette-Guérin (BCG). This assay would be useful both for detection of the M. tuberculosis complex and for differentiation of M. bovis BCG from pathogenic M. tuberculosis complex species.

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Year:  2010        PMID: 20943867      PMCID: PMC3008458          DOI: 10.1128/JCM.00872-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  20 in total

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Review 5.  Molecular methods for Mycobacterium tuberculosis strain typing: a users guide.

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6.  BCG osteomyelitis caused by the BCG Tokyo strain and confirmed by molecular method.

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7.  Occurrence and stability of insertion sequences in Mycobacterium tuberculosis complex strains: evaluation of an insertion sequence-dependent DNA polymorphism as a tool in the epidemiology of tuberculosis.

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8.  Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization.

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Review 9.  Specific immune-based diagnosis of tuberculosis.

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10.  RIDOM: comprehensive and public sequence database for identification of Mycobacterium species.

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  6 in total

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3.  Infected Aortic Aneurysm caused by Mycobacterium bovis after Intravesical Bacillus Calmette-Guérin Treatment for Bladder Cancer.

Authors:  Eun Young Nam; Sun Hee Na; Se Yong Kim; Doran Yoon; Chung-Jong Kim; Kyoung Un Park; Seung-Kee Min; Sang Eun Lee; Pyoeng Gyun Choe
Journal:  Infect Chemother       Date:  2015-12-30

4.  First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay.

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Journal:  PLoS Negl Trop Dis       Date:  2017-05-05

5.  Simultaneous Detection of Bovine Rotavirus, Bovine Parvovirus, and Bovine Viral Diarrhea Virus Using a Gold Nanoparticle-Assisted PCR Assay With a Dual-Priming Oligonucleotide System.

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6.  Validation of a real-time PCR assay for the molecular identification of Mycobacterium tuberculosis.

Authors:  Mariana L Sales; Antônio Augusto Fonseca Júnior; Lívia Orzil; Andrea Padilha Alencar; Marcio Roberto Silva; Marina Azevedo Issa; Paulo Martins Soares Filho; Andrey Pereira Lage; Marcos Bryan Heinemann
Journal:  Braz J Microbiol       Date:  2015-03-04       Impact factor: 2.476

  6 in total

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