Literature DB >> 20939826

Current strategies for probing substrate specificity of proteases.

M Poreba1, M Drag.   

Abstract

In this review we describe in detail the available technologies used for investigating the substrate specificity of proteases. Critical comparison of the available detection methods and their choice for certain type of screening is discussed. We present successful strategies along with appropriate examples for the design and synthesis of combinatorial libraries of substrates using both chemical and biological approaches. Proteomic tools for the identification of natural substrates of proteases are also discussed.

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Year:  2010        PMID： 20939826     DOI： 10.2174/092986710793205381

Source DB:  PubMed          Journal:  Curr Med Chem        ISSN： 0929-8673            Impact factor:   4.530


  23 in total

1.  Design of ultrasensitive probes for human neutrophil elastase through hybrid combinatorial substrate library profiling.

Authors:  Paulina Kasperkiewicz; Marcin Poreba; Scott J Snipas; Heather Parker; Christine C Winterbourn; Guy S Salvesen; Marcin Drag
Journal:  Proc Natl Acad Sci U S A       Date:  2014-02-03       Impact factor: 11.205

Review 2.  New approaches for dissecting protease functions to improve probe development and drug discovery.

Authors:  Edgar Deu; Martijn Verdoes; Matthew Bogyo
Journal:  Nat Struct Mol Biol       Date:  2012-01-05       Impact factor: 15.369

Review 3.  Small Molecule Active Site Directed Tools for Studying Human Caspases.

Authors:  Marcin Poreba; Aleksandra Szalek; Paulina Kasperkiewicz; Wioletta Rut; Guy S Salvesen; Marcin Drag
Journal:  Chem Rev       Date:  2015-11-09       Impact factor: 60.622

4.  Counter Selection Substrate Library Strategy for Developing Specific Protease Substrates and Probes.

Authors:  Marcin Poreba; Rigmor Solberg; Wioletta Rut; Ngoc Nguyen Lunde; Paulina Kasperkiewicz; Scott J Snipas; Marko Mihelic; Dusan Turk; Boris Turk; Guy S Salvesen; Marcin Drag
Journal:  Cell Chem Biol       Date:  2016-07-28       Impact factor: 8.116

5.  Synthesis of a HyCoSuL peptide substrate library to dissect protease substrate specificity.

Authors:  Marcin Poreba; Guy S Salvesen; Marcin Drag
Journal:  Nat Protoc       Date:  2017-09-21       Impact factor: 13.491

6.  An amino acid substitution inhibits specialist herbivore production of an antagonist effector and recovers insect-induced plant defenses.

Authors:  Eric A Schmelz; Alisa Huffaker; Mark J Carroll; Hans T Alborn; Jared G Ali; Peter E A Teal
Journal:  Plant Physiol       Date:  2012-09-24       Impact factor: 8.340

Review 7.  Assessing the range of enzymatic and oxidative tunability for biosensor design.

Authors:  Hattie C Schunk; Derek S Hernandez; Mariah J Austin; Kabir S Dhada; Adrianne M Rosales; Laura J Suggs
Journal:  J Mater Chem B       Date:  2020-04-29       Impact factor: 6.331

8.  Extensive peptide and natural protein substrate screens reveal that mouse caspase-11 has much narrower substrate specificity than caspase-1.

Authors:  Monica L Gonzalez Ramirez; Marcin Poreba; Scott J Snipas; Katarzyna Groborz; Marcin Drag; Guy S Salvesen
Journal:  J Biol Chem       Date:  2018-02-06       Impact factor: 5.157

Review 9.  Caspase substrates and inhibitors.

Authors:  Marcin Poreba; Aleksandra Strózyk; Guy S Salvesen; Marcin Drag
Journal:  Cold Spring Harb Perspect Biol       Date:  2013-08-01       Impact factor: 10.005

10.  Activity-based probes for rhomboid proteases discovered in a mass spectrometry-based assay.

Authors:  Oliver Vosyka; Kutti R Vinothkumar; Eliane V Wolf; Arwin J Brouwer; Rob M J Liskamp; Steven H L Verhelst
Journal:  Proc Natl Acad Sci U S A       Date:  2013-01-28       Impact factor: 11.205
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