On the isomerisation of cefozopran in solution.

In this study, we first described the two configurations of cephalosporins by cefozopran, then determined their molecular structure and finally evaluated the stability and biological activity of the two configurations. Our results showed that cefozopran existed as two different configurations in the aqueous solution with acetonitrile. Using mass spectrometry with liquid chromatography, nuclear magnetic resonance with liquid chromatography and optical rotation detection technology, we determined the spatial structures of both configurations and the detailed mechanism for change. By molecular docking and determining their antimicrobial activities, we showed that the biological activity of cis-isomer was stronger than that of trans-isomer.