Literature DB >> 20926084

Hydrophilic interaction chromatography of intact, soluble proteins.

Tim Tetaz1, Simon Detzner, Arno Friedlein, Birgit Molitor, Jean-Luc Mary.   

Abstract

The separation of intact proteins by means of Hydrophilic Interaction Chromatography (HILIC) was demonstrated with human apoA-I, recombinant human apoM, and equine cytochrome C. Five different commercially available HILIC columns were compared. Using one of these columns, different glycosylated isoforms of apoM were separated from each other and from the aglyco-form.
Copyright © 2010 Elsevier B.V. All rights reserved.

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Year:  2010        PMID： 20926084     DOI： 10.1016/j.chroma.2010.09.027

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  8 in total

1.  Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.

Authors:  Zhaorui Zhang; Zhen Wu; Mary J Wirth
Journal:  J Chromatogr A       Date:  2013-06-06       Impact factor: 4.759

2.  Online Hydrophobic Interaction Chromatography-Mass Spectrometry for Top-Down Proteomics.

Authors:  Bifan Chen; Ying Peng; Santosh G Valeja; Lichen Xiu; Andrew J Alpert; Ying Ge
Journal:  Anal Chem       Date:  2016-01-14       Impact factor: 6.986

3.  Retention Study of Flavonoids Under Different Chromatographic Modes.

Authors:  Aleksandra Sentkowska; Magdalena Biesaga; Krystyna Pyrzynska
Journal:  J Chromatogr Sci       Date:  2015-12-13       Impact factor: 1.618

Review 4.  Different Stationary Phase Selectivities and Morphologies for Intact Protein Separations.

Authors:  A Astefanei; I Dapic; M Camenzuli
Journal:  Chromatographia       Date:  2016-09-23       Impact factor: 2.044

5.  Poly(acrylamide-co-N,N'-methylenebisacrylamide) Monoliths for High-Peak-Capacity Hydrophilic-Interaction Chromatography-High-Resolution Mass Spectrometry of Intact Proteins at Low Trifluoroacetic Acid Content.

Authors:  Marta Passamonti; Chiem de Roos; Peter J Schoenmakers; Andrea F G Gargano
Journal:  Anal Chem       Date:  2021-11-22       Impact factor: 6.986

6.  Protein-induced conformational change in glycans decreases the resolution of glycoproteins in hydrophilic interaction liquid chromatography.

Authors:  Charles R Bupp; Cameron Schwartz; Bingchuan Wei; Mary J Wirth
Journal:  J Sep Sci       Date:  2021-03-31       Impact factor: 3.645

7.  Monitoring antigenic protein integrity during glycoconjugate vaccine synthesis using capillary electrophoresis-mass spectrometry.

Authors:  Sara Tengattini; Elena Domínguez-Vega; Caterina Temporini; Marco Terreni; Govert W Somsen
Journal:  Anal Bioanal Chem       Date:  2016-07-02       Impact factor: 4.142

8.  Capillary HILIC-MS: A New Tool for Sensitive Top-Down Proteomics.

Authors:  Andrea F G Gargano; Liana S Roca; Ryan T Fellers; Max Bocxe; Elena Domínguez-Vega; Govert W Somsen
Journal:  Anal Chem       Date:  2018-05-18       Impact factor: 6.986
  8 in total

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