Literature DB >> 20923139

The aging-associated enzyme CLK-1 is a member of the carboxylate-bridged diiron family of proteins.

Rachel K Behan1, Stephen J Lippard.   

Abstract

The aging-associated enzyme CLK-1 is proposed to be a member of the carboxylate-bridged diiron family of proteins. To evaluate this hypothesis and characterize the protein, we expressed soluble mouse CLK-1 (MCLK1) in Escherichia coli as a heterologous host. Using Mössbauer and EPR spectroscopy, we established that MCLK1 indeed belongs to this protein family. Biochemical analyses of the in vitro activity of MCLK1 with quinone substrates revealed that NADH can serve directly as a reductant for catalytic activation of dioxygen and substrate oxidation by the enzyme, with no requirement for an additional reductase protein component. The direct reaction of NADH with a diiron-containing oxidase enzyme has not previously been encountered for any member of the protein superfamily.

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Year:  2010        PMID： 20923139      PMCID： PMC2976817          DOI： 10.1021/bi101475z

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  29 in total

1.  Reversal of the mitochondrial phenotype and slow development of oxidative biomarkers of aging in long-lived Mclk1+/- mice.

Authors:  Jérôme Lapointe; Zaruhi Stepanyan; Eve Bigras; Siegfried Hekimi
Journal:  J Biol Chem       Date:  2009-05-28       Impact factor: 5.157

2.  Mouse homologue of coq7/clk-1, longevity gene in Caenorhabditis elegans, is essential for coenzyme Q synthesis, maintenance of mitochondrial integrity, and neurogenesis.

Authors:  D Nakai; S Yuasa; M Takahashi; T Shimizu; S Asaumi; K Isono; T Takao; Y Suzuki; H Kuroyanagi; K Hirokawa; H Koseki; T Shirsawa
Journal:  Biochem Biophys Res Commun       Date:  2001-11-30       Impact factor: 3.575

3.  Evolutionary conservation of the clk-1-dependent mechanism of longevity: loss of mclk1 increases cellular fitness and lifespan in mice.

Authors:  Xingxing Liu; Ning Jiang; Bryan Hughes; Eve Bigras; Eric Shoubridge; Siegfried Hekimi
Journal:  Genes Dev       Date:  2005-09-29       Impact factor: 11.361

4.  Yeast Clk-1 homologue (Coq7/Cat5) is a mitochondrial protein in coenzyme Q synthesis.

Authors:  T Jonassen; M Proft; F Randez-Gil; J R Schultz; B N Marbois; K D Entian; C F Clarke
Journal:  J Biol Chem       Date:  1998-02-06       Impact factor: 5.157

5.  Mössbauer and EPR studies of the binuclear iron center in ribonucleotide reductase from Escherichia coli. A new iron-to-protein stoichiometry.

Authors:  J B Lynch; C Juarez-Garcia; E Münck; L Que
Journal:  J Biol Chem       Date:  1989-05-15       Impact factor: 5.157

6.  A family of diiron monooxygenases catalyzing amino acid beta-hydroxylation in antibiotic biosynthesis.

Authors:  Thomas M Makris; Mrinmoy Chakrabarti; Eckard Münck; John D Lipscomb
Journal:  Proc Natl Acad Sci U S A       Date:  2010-08-16       Impact factor: 11.205

7.  Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.

Authors:  Rieko Oshima; Shinya Fushinobu; Fei Su; Li Zhang; Naoki Takaya; Hirofumi Shoun
Journal:  J Mol Biol       Date:  2004-09-03       Impact factor: 5.469

Review 8.  Carbanion versus hydride transfer mechanisms in flavoprotein-catalyzed dehydrogenations.

Authors:  Paul F Fitzpatrick
Journal:  Bioorg Chem       Date:  2004-06       Impact factor: 5.275

9.  Properties of ubiquinol oxidase reconstituted from ubiquinol-cytochrome c reductase, cytochrome c and cytochrome c oxidase.

Authors:  R J Diggens; C I Ragan
Journal:  Biochem J       Date:  1982-02-15       Impact factor: 3.857

10.  Mitochondrial recycling of ascorbic acid from dehydroascorbic acid: dependence on the electron transport chain.

Authors:  Xia Li; Charles E Cobb; James M May
Journal:  Arch Biochem Biophys       Date:  2002-07-01       Impact factor: 4.013
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  20 in total

1.  Yeast Coq9 controls deamination of coenzyme Q intermediates that derive from para-aminobenzoic acid.

Authors:  Cuiwen H He; Dylan S Black; Theresa P T Nguyen; Charles Wang; Chandra Srinivasan; Catherine F Clarke
Journal:  Biochim Biophys Acta       Date:  2015-05-23

2.  Toward functional carboxylate-bridged diiron protein mimics: achieving structural stability and conformational flexibility using a macrocylic ligand framework.

Authors:  Loi H Do; Stephen J Lippard
Journal:  J Am Chem Soc       Date:  2011-06-17       Impact factor: 15.419

3.  Restoring de novo coenzyme Q biosynthesis in Caenorhabditis elegans coq-3 mutants yields profound rescue compared to exogenous coenzyme Q supplementation.

Authors:  Fernando Gomez; Ryoichi Saiki; Randall Chin; Chandra Srinivasan; Catherine F Clarke
Journal:  Gene       Date:  2012-06-23       Impact factor: 3.688

Review 4.  Cyanobacterial alkane biosynthesis further expands the catalytic repertoire of the ferritin-like 'di-iron-carboxylate' proteins.

Authors:  Carsten Krebs; J Martin Bollinger; Squire J Booker
Journal:  Curr Opin Chem Biol       Date:  2011-04       Impact factor: 8.822

Review 5.  Biochemistry of Mitochondrial Coenzyme Q Biosynthesis.

Authors:  Jonathan A Stefely; David J Pagliarini
Journal:  Trends Biochem Sci       Date:  2017-09-17       Impact factor: 13.807

Review 6.  Evolution of strategies to prepare synthetic mimics of carboxylate-bridged diiron protein active sites.

Authors:  Loi H Do; Stephen J Lippard
Journal:  J Inorg Biochem       Date:  2011-09-14       Impact factor: 4.155

7.  Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis.

Authors:  Danielle C Lohman; Farhad Forouhar; Emily T Beebe; Matthew S Stefely; Catherine E Minogue; Arne Ulbrich; Jonathan A Stefely; Shravan Sukumar; Marta Luna-Sánchez; Adam Jochem; Scott Lew; Jayaraman Seetharaman; Rong Xiao; Huang Wang; Michael S Westphall; Russell L Wrobel; John K Everett; Julie C Mitchell; Luis C López; Joshua J Coon; Liang Tong; David J Pagliarini
Journal:  Proc Natl Acad Sci U S A       Date:  2014-10-22       Impact factor: 11.205

8.  Mechanistic studies of reactions of peroxodiiron(III) intermediates in T201 variants of toluene/o-xylene monooxygenase hydroxylase.

Authors:  Woon Ju Song; Stephen J Lippard
Journal:  Biochemistry       Date:  2011-05-19       Impact factor: 3.162

9.  Aging-associated enzyme human clock-1: substrate-mediated reduction of the diiron center for 5-demethoxyubiquinone hydroxylation.

Authors:  Tsai-Te Lu; Seung Jae Lee; Ulf-Peter Apfel; Stephen J Lippard
Journal:  Biochemistry       Date:  2013-03-20       Impact factor: 3.162

10.  Coq6 is responsible for the C4-deamination reaction in coenzyme Q biosynthesis in Saccharomyces cerevisiae.

Authors:  Mohammad Ozeir; Ludovic Pelosi; Alexandre Ismail; Caroline Mellot-Draznieks; Marc Fontecave; Fabien Pierrel
Journal:  J Biol Chem       Date:  2015-08-10       Impact factor: 5.157
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