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Literature DB >> 20887859

Site-specific biomolecule labeling with gold clusters.

Christopher J Ackerson¹, Richard D Powell, James F Hainfeld.

Abstract

Site-specific labeling of biomolecules in vitro with gold clusters can enhance the information content of electron cryomicroscopy experiments. This chapter provides a practical overview of well-established techniques for forming biomolecule/gold cluster conjugates. Three bioconjugation chemistries are covered: linker-mediated bioconjugation, direct gold-biomolecule bonding, and coordination-mediated bonding of nickel(II) nitrilotriacetic acid (NTA)-derivatized gold clusters to polyhistidine (His)-tagged proteins.

Entities: Chemical Disease Gene Species

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Year: 2010 PMID： 20887859 PMCID： PMC3568671 DOI： 10.1016/S0076-6879(10)81009-2

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

68 in total

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2. BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip.

Authors: L Nieba; S E Nieba-Axmann; A Persson; M Hämäläinen; F Edebratt; A Hansson; J Lidholm; K Magnusson; A F Karlsson; A Plückthun
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5. Assembly of nanoparticle-protein binding complexes: from monomers to ordered arrays.

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Journal: J Biol Chem Date: 2005-09-19 Impact factor: 5.157

7. Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy.

Authors: Jonathan Z Sexton; Christopher J Ackerson
Journal: J Phys Chem C Nanomater Interfaces Date: 2010-09-30 Impact factor: 4.126

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9. Localization of the N-terminal domain of the low density lipoprotein receptor.

Authors: H Jeon; G G Shipley
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10. Crystal structure of the gold nanoparticle [N(C8H17)4][Au25(SCH2CH2Ph)18].

Authors: Michael W Heaven; Amala Dass; Peter S White; Kennedy M Holt; Royce W Murray
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20 in total

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5. Progress toward clonable inorganic nanoparticles.

Authors: Thomas W Ni; Lucian C Staicu; Richard S Nemeth; Cindi L Schwartz; David Crawford; Jeffrey D Seligman; William J Hunter; Elizabeth A H Pilon-Smits; Christopher J Ackerson
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6. High-affinity gold nanoparticle pin to label and localize histidine-tagged protein in macromolecular assemblies.

Authors: Kelsey C Anthony; Changjiang You; Jacob Piehler; Daniel A Pomeranz Krummel
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7. Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy.

Authors: Ramakrishnan B Kumar; Lin Zhu; Hans Hebert; Caroline Jegerschöld
Journal: J Vis Exp Date: 2017-03-05 Impact factor: 1.355

8. Use of single chain antibody derivatives for targeted drug delivery.

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9. Site-specific covalent labeling of large RNAs with nanoparticles empowered by expanded genetic alphabet transcription.

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Journal: Proc Natl Acad Sci U S A Date: 2020-08-31 Impact factor: 11.205

Review 10. Development and application of STEM for the biological sciences.

Authors: Alioscka A Sousa; Richard D Leapman
Journal: Ultramicroscopy Date: 2012-05-18 Impact factor: 2.689