Development of a microfluidic confocal fluorescence detection system for the hyphenation of nano-LC to on-line biochemical assays.

One way to profile complex mixtures for receptor affinity is to couple liquid chromatography (LC) on-line to biochemical detection (BCD). A drawback of this hyphenated screening approach is the relatively high consumption of sample, receptor protein and (fluorescently labeled) tracer ligand. Here, we worked toward minimization of sample and reagent consumption, by coupling nano-LC on-line to a light-emitting diode (LED) based capillary confocal fluorescence detection system capable of on-line BCD with low-flow rates. In this fluorescence detection system, a capillary with an extended light path (bubble cell) was used as a detection cell in order to enhance sensitivity. The technology was applied to a fluorescent enhancement bioassay for the acetylcholine binding protein, a structural analog of the extracellular ligand-binding domain of neuronal nicotinic acetylcholine receptors. In the miniaturized setup, the sensitive and low void volume LED-induced confocal fluorescence detection system operated in flow injection analysis mode allowing the measurement of IC(50) values, which were comparable with those measured by a conventional plate reader bioassay. The current setup uses 50 nL as injection volume with a carrier flow rate of 400 nL/min. Finally, coupling of the detection system to gradient reversed-phase nano-LC allowed analysis of mixtures in order to identify the bioactive compounds present by injecting 10 nL of each mixture.

Introduction

In search of bioactive compounds, many exogenous natural products were demonstrated to have valuable starting points for medicinal chemistry. For this reason, there is a continuous effort to capture the available biodiversity, but for some natural products, the available extracts are very scarce, and consequently, only limited amounts are available for analysis. Liquid chromatography (LC) is the separation workhorse in the process of screening for bioactive compounds in complex mixtures. After separation, fractionation, and evaporation, the bioactivity of each fraction is analyzed. This traditional dereplication process is usually associated with many bottlenecks as it is laborious and time consuming, and often multiple iterative cycles are needed to identify and obtain the pure bioactive compounds. This requires large amounts of starting material.

By coupling LC to on-line biochemical detection (BCD) and mass spectrometry (MS), bioactive mixtures can be screened more rapidly and comprehensively as bioactive compounds are simultaneously identified and unknowns may be structurally elucidated [1-6]. When applied for the screening of receptor ligands, the LC effluent is continuously mixed with the assay compounds consisting of target receptor and a fluorescent tracer ligand that has an enhanced fluorescence yield in the receptor’s binding pocket [7, 8]. A fluorescence detector is then used for the BCD readout. Even though less amount of sample is needed for successful on-line BCD than for traditional dereplication processes, in many cases, still substantial amounts are required. Nano-LC offers a solution to this problem from the separation’s perspective, but needs the on-line fluorescence BCD part also to be miniaturized.

As microfluidic technologies utilize highly miniaturized and complex chip-based systems, there is a general demand for miniaturized detection methods that are sensitive and selective, in particular also for fluorescence detection [9]. An example of this is laser-induced fluorescence detection [10], but costs for adequate lasers are high and excitation wavelengths are either fixed or only have a limited tunable wavelength range [11, 12]. LED-induced fluorescence has been utilized in specific cases for, e.g., cell based analysis [13] and has the advantage of easily interchangeable LEDs for a wider range of wavelengths. Other techniques rely on non-flow-based or non-LC flow-based detection in confocal fluorescence mode [14]. Often, microfluidic chip-based setups are used for both incubation/chemical reaction steps and for fluorescence readout [15-17]. A disadvantage in these setups can be the restriction in freely changing the incubation unit and detection readout, which can be overcome by separating the incubation chamber and the detection cell. As a non-chip-based detection cell, a stripped fused silica capillary is a good option [18], although the flow path in narrow capillaries is very narrow, which limits the sensitivity. Even when operated in confocal mode, the sensitivity is compromised unless the confocal optics are of high quality and the positioning of the confocal point is very accurate, which means that expensive setups are necessary. Implementation of a bubble cell capillary is able to overcome this limitation.

This study presents the development of a microfluidic LED-based confocal fluorescence detection (CFD) system, with a bubble cell capillary as detection cell, capable of monitoring fluorescence bioassays in a continuous low-flow format. In this way, reduction of reagent and sample consumption in an on-line biochemical analysis format can be achieved by decreasing the overall flow rates for the fluorescence-based on-line BCD. An advantage of the new microfluidic CFD with respect to other low-flow rate fluorescence detection methods is the adaptation for on-line BCD at very low-flow rates in combination with nano-LC. The chromatographic part of the system utilizes nano-LC separations at sub-μL/min flow rates, which was coupled on-line to the microfluidic CFD. The detection system consisted of a LED, excitation and emission filters, a confocal lens, a photomultiplier tube, and a bubble cell capillary acting as a detector cell (Fig. 1). To demonstrate the applicability of the system developed, an on-line BCD methodology with a fluorescence enhancement bioassay of acetylcholine binding protein (AChBP) as receptor in conjunction with the in-house made fluorescent tracer ligand (E)-3-(3-(4-diethylamino-2-hydroxybenzylidene)-3,4,5,6-tetrahydropyridin-2-yl)pyridine (DAHBA) [8] was applied. As a biochemical incubation chamber for the miniaturized AChBP bioassay, a microfluidic chip was employed. This chip was similar to a previously developed chip, which was used for a miniaturized enzymatic on-line BCD utilizing mass spectrometry as readout [19]. Since combining flows in the nanoliter per minute range often leads to laminar flows, special considerations of mixing procedures are required [20, 21]. Therefore, we used a microfluidic chip for efficient mixing of the bioassay flows [19]. The chip provides both efficient mixing of the nano-LC effluent and the bioassay carrier flow and does so in a convenient and robust manner directing both flows to the CFD. When compared with traditional on-line bioaffinity systems [8, 22], the current setup not only allowed injection volumes of approximately a thousand times smaller but also decreased the overall flow rate of the bioassay ~30 times. This enabled the use of more convenient 2.5 mL syringes for continuous-flow bioassay delivery instead of 100 mL superloops operated by LC pumps.

Fig. 1

Schematic view of the complete on-line microfluidic CFD system. The bioassay carrier solution was delivered by a syringe pump (5 μL/min) and mixed with the nano-LC effluent (400 nL/min) in the 4-μL reaction chamber of the microfluidic chip. A detailed photographic picture of the microfluidic chip can be found in de Boer et al. [19]. The outlet of the chip was hyphenated to the bubble cell capillary where fluorescence was detected by the PMT of the microfluidic CFD. A detailed description of the system is given in the “Experimental” section

AChBP is considered an analog for the ligand-binding domain of neuronal nicotinic acetylcholine receptors, which are potential pharmaceutical targets for Alzheimer’s disease, Parkinson’s disease, pain relief, anxiety, epilepsy, and several cognitive deficits [23-27]. These facts underline the importance of this target protein for drug discovery purposes.

Experimental

Chemical and biological reagents

AChBP (from snail species Lymnaea stagnalis) was expressed from Baculovirus using the pFastbac I vector in Sf9 insect cells and purified from the medium as described by Celie et al. [23]. The tracer ligand DAHBA, (E)-3-(3-(4-diethylamino-2-hydroxybenzylidene)-3,4,5,6-tetrahydropyridin-2-yl)pyridine, was synthesized in-house [8]. KH2PO4, Na2HPO4, NaCl, trizma base, hydrochloric acid, formic acid (FA), rhodamine-110, 5% dimethyldichlorosilane (DMDCS) in toluene, and HPLC grade acetonitrile (99.9%) were purchased from Sigma-Aldrich, Zwijndrecht, The Netherlands. ELISA blocking reagent was purchased from Roche, Mannheim, Germany. LC-MS grade methanol (MeOH; 99.95%) was purchased from Biosolve, Valkenswaard, The Netherlands. HPLC grade water was produced using a Milli-Q purification system, Millipore, Amsterdam, The Netherlands. All ligands used for evaluating and validating the system were synthesized by the VU University Medicinal Chemistry department. The synthetic routes to compounds 1 and 7 were recently published by Kool and de Kloe et al. [8]. The synthetic route of compound 5 was recently published by Eggink et al. [28], referred to as 4-APC. The binding of compounds 2, 3, 4, 6, 8, and 9 to AChBP was recently studied by De Kloe and Retra et al. [29]. The structures of all compounds including the tracer ligand DAHBA can be found in Fig. 2.

Fig. 2

Structures of the compounds/ligands used for validation and evaluation of the on-line microfluidic CFD bioaffinity system

Instrumentation

The complete system consisted of a nano-LC unit including a UV detector, a microfluidic chip as biochemical reactor, and a microfluidic LED-based CFD (Fig. 1).

Nano-LC and solvent delivery system

The Ultimate nano-LC system with a Famos autosampler and UV detector was from LC Packings, Amsterdam, The Netherlands. The system was operated at 400 nL/min. Mobile phase solvent A consisted of water/MeOH 95:5 and 0.1% FA; solvent B of water/MeOH 5:95; and 0.1% FA. Sample volumes ranging from 10 to 50 nL were injected into the analytical capillary column (nano-LC; 150 mm × 100 μm i.d.) packed in-house with Waters Xbridge C18 particles (5 μm, 135 Å pore diameter; Waters, Milford, MA, USA). For mixture analysis, gradient elution was applied running 15 min isocratic at 0% solvent B, then rising to 75% solvent B in 45 min. The column exit was coupled via a low void volume Z-shaped capillary (3 nL UV-detector cell) to the microfluidic chip and the CFD.

Microfluidic chip

The microfluidic chip and the 4515 chip holder were produced by Micronit Microfluidics, Enschede, The Netherlands, as described earlier [19] and shown schematically in Fig. 1 with all the interconnecting capillaries. Microchip ferrules were purchased from Upchurch Scientific, Oak Harbor, USA. The syringe pump was from Harvard Apparatus, Holliston, USA. Syringes were purchased from Hamilton AG, Bonaduz, Switzerland.

A schematic representation of the microfluidic chip is shown in Fig. 3. The 45 × 15 × 2.2 mm chip consisted of two borofloat glass 33/D263 layers with a 457 mm long × 125 μm wide and 70 μm deep open tubular microreactor of 4 μL, comprising the total volume of the chip’s channel. The reactor had two inlets and one outlet. One inlet was used to connect the nano-LC carrier flow; the other inlet was used to infuse the AChBP and tracer ligand DAHBA at a flow rate of 5 μL/min by a syringe pump. The chip outlet was connected to the microfluidic CFD.

Fig. 3

The microfluidic chip, which comprises the bioaffinity analysis part of the system. AChBP and DAHBA are infused to the chip at 5 μL/min (1) together with the nano-LC effluent containing potential ligands (2). In the 4 μL open tubular microreactor of the chip, DAHBA is displaced by eluting ligands from the analytical column (3) resulting in a decrease in fluorescence (4) as detected by the microfluidic CFD

All fused silica capillaries used for connections to and from the chip were purchased from BGB Analytik AG, Schloßböckelheim, Germany. A 300 mm × 20 μm internal diameter (i.d.) capillary connected the nano-LC system to the chip. The capillary connecting the syringe pump to the chip was deactivated by the supplier with 1,3-diphenyl-1,1,3,3-tetramethyldisilazane and was 1,500 mm × 50 μm i.d. The outlet capillary to the bubble cell of the CFD was 1,000 mm × 50 μm i.d. The microchip, the 20 μm i.d. capillary, and the bubble cell capillary were deactivated with 5% DMDCS in toluene as follows: The chip and the tubing were flushed with acetonitrile (5 min), 5% DMDCS (5 min), acetonitrile (5 min), and MeOH (5 min) at a flow rate of 100 μL/min from a syringe pump.

Microfluidic LED-based confocal fluorescence detector

The microfluidic CFD is shown in detail in Fig. 1. The flow cell of the CFD consisted of a 150 μm i.d. extended light path “bubble cell” with 50 μm i.d. connecting capillaries (no. G1600-64232) and was purchased from Agilent Technologies, Amstelveen, The Netherlands. The LED (465 nm, 45 mW, 350 mA; no. ELJ-465-211) was from Roithner Lasertechnik, Vienna, Austria. The quartz microscope objective (20 × 0.65, working distance 2.0 mm; no. 03-0202) was from Partec Optics, Münster, Germany. The photomultiplier tube, incorporated in a photosensor module (185- to 650 nm spectral response; no. H9656-03) was from Hamamatsu, Shuzuoka, Japan. Focusing lenses were from Bernhard Halle Nachfl. GmbH, Berlin, Germany. The dichroic mirror (506 nm; no. FF506-Di02-25) and bandpass filters (465 nm and 520 nm; no. FF01-465/30-25 and FF01-520/35-25) were from Semrock, Rochester, USA.

In the CFD, the bubble cell serves as the actual flow-through detector cell. Light emanating from the high-intensity LED passed through the 465 nm single bandpass filter, after which it was collimated by a lens and reflected under 90° by the dichroic mirror. The filtered light was subsequently focused into the center of the 150 μm bubble cell by the 20× quartz microscope objective. Emitted light then passed the same dichroic mirror, a focusing lens, and the 520 nm single bandpass filter and was subsequently detected by the photomultiplier tube. The LASER (473 nM, 10 mW, DPSS RLTMBL) used in the initial setup was also from Roithner Lasertechnik.

Hardware optimization and chemical evaluation

Signal-to-noise determinations of the microfluidic CFD at different settings

At each photomultiplier tube (PMT) control voltage (8.0 to 10.0 V at 0.5 V increments tested) and four different LED input currents (from 200 to 350 mA at 50 mA increments), baselines were recorded at a frequency of 10 Hz for 1 min, while infusing a water/MeOH 50:50 solution to the microfluidic CFD with the syringe pump operated at 5 μL/min. The noise of the CFD system at each specific condition was calculated as two times the standard deviation of the 600 data points measured. In order to obtain a fluorescence signal, the above-described measurements were repeated with infusion of rhodamine-110 at a concentration of 500 pM. The measured 600 data points at each specific condition were averaged. Thus, the signal-to-noise ratio for 500 pM rhodamine-110 at five different PMT control voltages and four LED input currents were calculated.

Linearity analysis of the microfluidic CFD

To evaluate linearity at the 20 conditions tested as described for the signal-to-noise calculation, rhodamine-110 solutions were infused with concentrations of 0, 0.5, 1.0, 2.5, 5.0, 10.0, and 25 nM in water/MeOH 50:50. The resulting rhodamine-110 baselines were averaged for data analysis.

Determination of IC50 values with the microfluidic CFD operated as on-line BCD

Nano-column based flow injection analysis (FIA) in a concentration–response manner allowed the determination of IC50 values of test compounds. These analyses were run in isocratic LC mode with 0% to 50% B in the mobile phase, depending on the lipophilicity of each investigated compound. Each compound eluted from the nano-column without retention.

AChBP biochemical bioassay

Fresh solutions of L. stagnalis Acetylcholine binding protein (AChBP) and the fluorescent tracer ligand DAHBA were made every day by dissolving 1 μg/mL AChBP and 40 nM DAHBA in the bioassay solution containing: 1 mM KH2PO4, 3 mM Na2HPO4, 0.16 mM NaCl, 20 mM trizma base/HCl at pH 7.5 and 400 μg/mL ELISA blocking reagent. The bioassay solution was housed in a 2.5 mL syringe. During the experiments, the AChBP/DAHBA bioassay solution in the syringe was kept at 4 °C. The on-line bioassay itself was performed at 22 °C. The syringe pump was operated at a flow rate of 5 μL/min. The resulting bioassay flow was mixed with the eluent from the nano-LC. Continuous in-flow incubation was allowed in the microfluidic chip and then followed by fluorescence detection with the microfluidic CFD.

Results and discussion

Evaluation of the on-line microfluidic confocal fluorescence detection system

Hardware setup

The microfluidic CFD setup initially contained a laser as light source. Although the light beam from the laser tested (from Roithner Lasertechnik, 473 nM, 10 mW, DPSS RLTMBL) was more focused, and therefore more powerful than the LED used in the rest of this study, it also had serious drawbacks. Besides the higher costs, lasers as a light source impose restrictions as they only allow one wavelength (or a limited range) per laser to be used. Furthermore, baseline stability and reproducibility of the tested laser were not adequate for our purposes. Due to these drawbacks, the LED clearly surpasses the tested laser as LEDs are cheap and available in a large variety of wavelengths. More importantly, they were found to produce a more stable and reproducible fluorescence detection system for our purposes. Therefore, a LED was incorporated into the system together with an additional collimating lens to focus the light of the LED. In order to evaluate and optimize sensitivity and performance of the detection, capillaries with 20, 50, 100, and 150 μm i.d. as well as two types of bubble cell capillaries were evaluated as detection cell by installing the capillaries into the CFD, followed by infusion of a rhodamine-110 solution. In these experiments, an excitation wavelength of 465 nm was applied. A detection window was created in the tested capillaries (except the bubble cell capillaries) by locally removing the polyimide coating. The 100 and 150 μm i.d. capillaries yielded higher fluorescence signals than the 20 or 50 μm i.d. capillaries, which rendered the fluorescence system rather insensitive due to the very limited light path. An evident drawback of the two larger i.d. capillaries as detector cells was the increased dead volume, which imposed problems upon implementation into the miniaturized flow system. Besides sensitivity issues, the 20 μm capillary was also very susceptible to clogging; therefore, capillaries with an i.d. smaller than 50 μm i.d. were not evaluated further. Next, two bubble cell capillaries [30] were evaluated. Both 50 and 75 μm i.d. capillaries (with a 150 and 200 μm bubble cell, respectively) were tested. Although the 75 μm i.d. capillary showed a slightly higher signal-to-noise ratio than the 50 μm i.d. capillary, the 50 μm i.d. bubble cell capillary was finally incorporated in the system. The 50 μm i.d. capillary part did not significantly increase the system dead volume (and thus band broadening), while the advantage of the bubble cell to provide an extended light path yielded a fluorescence readout with a 5.6 times increased sensitivity compared with the 50 μm i.d. capillary without a bubble cell. This was in good agreement with the six times’ increase in sensitivity as given by the supplier.

Electronic settings (LED intensity and PMT control voltage)

The final microfluidic CFD hardware setup was subsequently evaluated by measuring the fluorescence of a blank and a 500 pM rhodamine-110 solution at 20 different combinations of LED and PMT settings. The results obtained are shown in Fig. S1 (Electronic supplementary material). The optimal signal-to-noise ratio was found at a power input to the LED of 350 mA and the control voltage of the PMT set at a value of 10 V, which were both the maximal settings. The signal-to-noise ratio of 500 pM rhodamine-110 was found to be 23 at these settings. Next, the linearity was evaluated by infusing different concentrations (0.5 to 25 nM) of rhodamine-110 at the optimal settings. From these results (Fig. S2: Electronic supplementary material), it was concluded that the system showed a high linearity at the optimal settings within the concentration range tested. These results also showed a dynamic range from 0.5 to 25 nM rhodamine-110 with an R 2 of 0.9998. Concentrations higher than 25 nM showed similar linearity, but could only be measured by decreasing the voltage to the PMT.

Biological evaluation and validation of the microfluidic confocal fluorescence detection system operated in on-line biochemical detection mode

Initial biochemical evaluation

The miniaturized on-line bioaffinity analysis part of the system is shown in Fig. 3. DAHBA and AChBP are continuously infused into the chip where the bioassay carrier solution is mixed continuously with the nano-LC eluent. Injected compounds elute from the nano-LC column to the chip where they are allowed to incubate with the miniaturized bioassay. In the incubation chamber of the chip, ligands displace DAHBA from the AChBP binding pocket. As this displacement results in a decreased fluorescence yield from DAHBA, it is detected by the microfluidic CFD as a negative peak in the on-line bioassay trace. The concentration (of 1 μg/mL) AChBP was chosen to allow a good assay window while maintaining optimal bioassay sensitivity. The concentration of tracer DAHBA used (40 nM) was slightly higher than its dissociation constant (K d) [8]. This is commonly done for ligand-binding assays. The optimal flow rate of the bioassay carrier solution was determined by varying the flow rate with incremental steps of 1 μL/min, starting at 1 μL/min. A stable baseline was observed at a minimal flow rate of 5 μL/min. At this flow rate, the syringe pump generated no pulses, nor did the 75% MeOH and 0.1% FA in the nano-LC effluent have any adverse effects on the bioassay. Higher flow rates of the bioassay carrier solution had a negative effect on the bioassay sensitivity because of dilution effects. During the initial biochemical evaluation and after test injections of AChBP ligands, a decline in assay sensitivity was observed. This was due to the adsorption of AChBP to the wall of the chip and bubble cell capillary, causing clogs, tailing effects, and an increased baseline. Therefore, a DMDCS deactivation procedure was applied to the chip, connecting capillaries and the bubble cell. With deactivated capillaries, no significant (increases in) band broadening were observed during a time period of 3 months. Also, a more stable baseline was observed and no clogging occurred anymore.

Biochemical validation

Nicotine, a typical ligand for AChBP, was used to evaluate intraday and interday repeatability of the complete system. Nicotine was injected at increasing concentrations, with a nano-LC carrier flow containing 0% eluent B, which allowed nicotine to elute from the column non-retained. A typical analysis result is shown in Fig. 4. The figure shows the displacement profile of 50 nL of nicotine injected in triplicate at 12 different concentrations in a serial dilution manner with a factor of two. Injecting different concentrations of nicotine showed negative peaks of which the negative peak heights represented the percentage of DAHBA displaced in the bioassay. Over 99% tracer DAHBA displacement by nicotine occurred at a concentration of 10 μM of nicotine in the bioassay. At full DAHBA displacement, injecting higher nicotine concentrations evidently did not yield higher negative signals, but only broader signals due to tailing effects. The figure also indicates the excellent repeatability for each concentration injected.

Fig. 4

Triplicate injections (50 nL) of increasing concentrations (100 nM to 10 mM, in-vial concentration) of the ligand nicotine in FIA mode. The observed negative peak heights were plotted against the concentrations inside the flow system to obtain an IC50 curve; see Fig. 5 for the IC50 curve of nicotine

Fig. 5

IC50 curves of six AChBP ligands. All ligands were injected in triplicate with an injection volume of 50 nL

The intraday repeatability was determined by measuring multiple IC50 curves during one measuring day using the same batch of bioassay constituents. Because ligands injected are diluted in the microfluidic BCD system, a dilution factor has to be used to correlate DAHBA displacements with the actual ligand concentrations in the on-line BCD part. Therefore, a dilution factor was calculated in order to determine IC50 values. This was done according to a mathematical analytical formula as presented previously [22, 31] (see also the Electronic supplementary material for the formula used and further explanation) and resulted in a dilution factor of 243, which was used further. Table 1 shows the analytical results. These results demonstrate that the system operated in a very repeatable manner (RSD = 1.5%). Furthermore, the average IC50 for nicotine as acquired from the IC50 curve shown in Fig. 5 (6.41), correlated well with results obtained by traditional biochemical assay formats (6.40; from Kool et al. [8]). The bioassay window was determined as the largest negative peak obtainable and was measured by injecting a very high concentration of nicotine. The bioassay window did not change markedly over a full measuring day. As seen in Fig. 4, representing a typical measuring result, a small baseline drift (of only 8% in this case) was observed during 7 h.

Table 1

Pharmacological validation by evaluation of the intraday repeatability and interday reproducibility

Parameter	1 (pIC50)	R 2	2 (pIC50)	R 2	3 (pIC50)	R 2
Intraday a	6.41 ± 0.02	0.9973	6.55 ± 0.02	0.9932	n.d.	n.d.
Interdayb	6.07 ± 0.01	0.9989	6.35 ± 0.01	0.9988	6.21 ± 0.02	0.9973

The numbers 1, 2, and 3 signify the respective measurements

aThe intraday repeatability was investigated by determining the IC50 value of nicotine two times in triplicate within 24 h

bFor the interday repeatability, the IC50 value of nicotine was determined three times in triplicate over the course of 3 days

The interday reproducibility was tested in essentially the same way as the intraday repeatability, but in this case, one IC50 curve was measured per day with every day a fresh solution of bioassay constituents for 3 days. These results are depicted in Table 1 and Fig. S3 (Electronic supplementary material) and clearly show the good reproducibility of the system (RSD = 2.3%). This was also the case for the bioassay window that did not show marked differences over the days; an average assay window (maximum obtainable S/N ratio) of 47.2 ± 1.7 was found. As the average IC50 found (IC50 of 6.38 ± 0.02) for nicotine (see Table 1) correlated well with results obtained by a traditional radioligand-binding assay (RBA; IC50 of 6.40 ± 0.01) [8] and low standard deviations were obtained, it was concluded that the methodology was not only accurate but also precise.

The results from both the repeatability as well as the reproducibility experiments allowed us to further validate the system by analyzing several ligands for a pharmacological comparison with traditional methodologies.

Pharmacological validation

To evaluate the biological sensitivity and validate the system pharmacologically, IC50 values of different ligands were measured the same way as done for the intraday repeatability and then compared with results from a traditional RBA. For this, five ligands including nicotine were analyzed. The structures of the ligands used can be found in Fig. 2. These quinuclidine-containing ligands were chosen because their binding to AChBP has been properly characterized [29]. After analysis of the ligands with both FIA on-line microfluidic BCD and with a traditional RBA, IC50 curves were constructed (see Fig. 5 for the on-line microfluidic BCD FIA measurements). Subsequently, a correlation plot was constructed by plotting the CFD pK i values to the pK i values of a traditional RBA. The R 2 of this plot then rates the applicability of the system to assess ligand binding. The model equation derived from Fig. 6 was y = 0.696x + 2.1455 with an R 2 = 0.9651 (Fig. 6). The correlation between results from our microfluidic system and traditional RBA demonstrated the excellent applicability of the microfluidic BCD system for IC50 determination of ligands. The system also proved to be very sensitive; the LOD of compound 1 (pK i of 8.71 ± 0.03) for example was 412 pM. In addition, compound 5 with a choline moiety that we used as derivatization reagent in previous work [28] was also investigated with the complete system. This was done because it was expected that this compound also had affinity toward the AChBP as the choline moiety represents an important pharmacophoric part for AChBP binding pocket. Indeed, compound 5 showed a low affinity (pK i of 5.45 ± 0.01) toward AChBP.

Fig. 6

Correlation plot of five typical ligands analyzed with both the traditional RBA and the on-line microfluidic BCD. An R 2 of 0.9651 was obtained

Proof-of-principle for mixture analysis of the microfluidic confocal fluorescence system

To show the potential of the microfluidic CFD for bioaffinity analysis of mixtures toward the AChBP, a mixture of eight ligands was separated by nano-LC followed by biochemical analysis with the miniaturized BCD format. A typical result obtained is shown in Fig. 7. For analysis of the mixture shown, only 10 nL of mixture was injected. The upper trace shows an UV readout at 254 nm of the eight ligands injected (100 pmol injected). The lower trace shows the simultaneously obtained bioaffinity trace. This trace clearly shows displacement of the DAHBA tracer ligand from the AChBP binding pocket upon eluting ligands, yielding negative peaks indicative of the total bioaffinity per ligand separated. The miniaturized system shows a very stable baseline, while bioaffinity peaks are obtained with very low band broadening effects. The system’s stability and low band broadening in the UV trace as well as in the bioassay enable a clear distinction between co-eluting compounds as can be seen for compounds 3, 7 and 8. The UV trace in Fig. 7 shows an excellent separation for these closely related analogs. These results also indicate that the miniaturized system could be used to establish structure–activity relationships, for this implies the evaluation of different substituents on one scaffold.

Fig. 7

On-line microfluidic BCD and UV analysis of a mixture containing eight AChBP ligands (10 nL injected). The upper trace shows the UV signal of the ligands. The lower trace shows the fluorescence bioaffinity signal, as obtained by the microfluidic CFD. Structures of the ligands are shown in Fig. 2

Both the IC50 measurements of pure compounds as well as the application of bioactive mixture analysis demonstrate that this miniaturized system is comparable in terms of sensitivity and stability with “non-miniaturized” on-line BCD systems where much higher flow rates and injection volumes are used [7, 8, 31]. Here, the additional advantage of our new miniaturized BCD technology is the use of very low sample volumes in a fluorescence-based detection setup. This renders the setup suitable for analysis of very scarce and/or precious samples, which is a topic of our current research efforts.

Conclusion

A microfluidic CFD was developed and successfully coupled to a miniaturized continuous-flow bioassay incorporating a nano-LC system as the separation technology. The implementation of a LED and a DMDCS deactivation procedure made the system stable, rugged, and very versatile for, e.g., on-line biochemical screening utilizing fluorescent tracer ligands with different fluorescence properties. Additional sensitivity was obtained by the implementation of a bubble cell capillary as detection cell. The presented system not only showed a very good stability and robustness but also decreased sample consumption a thousand fold as compared with conventional on-line systems. By miniaturizing both the overall flow rates of the system from the micro- to the nano-scale and the dimensions used in the flow system, a miniaturized continuous-flow system was obtained. Here, the enormous reduction in consumption of sample and biochemical reagents provides for a larger range in possible drug targets for on-line bioactivity screening, which are for example difficult to produce or obtain in larger quantities. Furthermore, the system allows chemical and simultaneous biochemical screening of scarce sample types only obtainable in very low volumes, such as precious natural extracts, animal venoms, cell, and tissue lysates. By miniaturizing, no stability and sensitivity were sacrificed when compared with conventional on-line systems.

Below is the link to the electronic supplementary material.

(PDF 82.8 kb)