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Literature DB >> 2086220

Evaluation of immunoblotting for the detection of Toxoplasma gondii immunoglobulin M antibodies.

C Verhofstede¹, L Van Renterghem, J Plum.

Abstract

Immunoblot analysis was used to detect human IgM antibodies to Toxoplasma gondii in 20 patients with recent toxoplasmosis, 30 immune individuals, 30 non-immune individuals, and 24 children less then two years old. Analysis of the IgM strips revealed that specific IgM antibodies detectable after a recent Toxoplasma gondii infection react with the same antigens as the 'natural' antibodies present in the sera of immune and non-immune individuals and in the sera of young children. These data indicate that immunoblotting is not useful as a reference method for Toxoplasma gondii IgM detection, and suggest that improvement of the specificity of IgM detection will remain difficult.

Entities: Disease Species

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Year: 1990 PMID： 2086220 DOI： 10.1007/BF01967387

Source DB: PubMed Journal: Eur J Clin Microbiol Infect Dis ISSN： 0934-9723 Impact factor: 3.267

12 in total

1. Comparison of six commercial enzyme linked immunosorbent assays for detecting IgM antibodies against Toxoplasma gondii.

Authors: C Verhofstede; L Van Renterghem; J Plum
Journal: J Clin Pathol Date: 1989-12 Impact factor: 3.411

2. Ability of enzyme-linked immunosorbent assays to detect early immunoglobulin G antibodies to Toxoplasma gondii.

Authors: C Verhofstede; L Sabbe; L Van Renterghem
Journal: Eur J Clin Microbiol Date: 1987-04 Impact factor: 3.267

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

4. [Natural antibodies against Toxoplasma].

Authors: G Desmonts; H Baufine-Ducrocq; P Couzineau; Y Peloux
Journal: Nouv Presse Med Date: 1974-06-15

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Specificity and sensitivity of the IgM capture immunoassay: studies of possible factors inducing false positive or false negative results.

Authors: M J Briantais; L Grangeot-Keros; J Pillot
Journal: J Virol Methods Date: 1984-08 Impact factor: 2.014

2. Improved serological diagnosis of Toxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique.

Authors: U Gross; T Roos; D Appoldt; J Heesemann
Journal: J Clin Microbiol Date: 1992-06 Impact factor: 5.948

2 in total

Evaluation of immunoblotting for the detection of Toxoplasma gondii immunoglobulin M antibodies.

1. Comparison of six commercial enzyme linked immunosorbent assays for detecting IgM antibodies against Toxoplasma gondii.

2. Ability of enzyme-linked immunosorbent assays to detect early immunoglobulin G antibodies to Toxoplasma gondii.

3. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

4. [Natural antibodies against Toxoplasma].

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

6. Specificity and sensitivity of the IgM capture immunoassay: studies of possible factors inducing false positive or false negative results.

7. Significance of specific immunoglobulin M in the chronological diagnosis of 38 cases of toxoplasmic lymphadenopathy.

8. A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.

9. Toxoplasma antigens recognized by naturally occurring human antibodies.

10. Indirect immunofluorescence in toxoplasmosis: frequency, nature and specificity of polar staining.

1. Improvement of immunoglobulin M capture immunoassay specificity: toxoplasma antibody detection method as a model.

2. Improved serological diagnosis of Toxoplasma gondii infection by detection of immunoglobulin A (IgA) and IgM antibodies against P30 by using the immunoblot technique.