BACKGROUND AIMS: Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation. METHODS: AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted. RESULTS: eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability. CONCLUSIONS: Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.
BACKGROUND AIMS: Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equineAF stem cells. We have isolated equineAF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation. METHODS:AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted. RESULTS: eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability. CONCLUSIONS:EquineAF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.
Authors: Eleonora Iacono; Marco Cunto; Daniele Zambelli; Francesca Ricci; Pier Luigi Tazzari; Barbara Merlo Journal: Vet Res Commun Date: 2012-02-12 Impact factor: 2.459