| Literature DB >> 20857262 |
Naoki Yamamoto1, Atsuhiro Tanikawa, Masayuki Horiguchi.
Abstract
We described the possibility of retinal regeneration using a novel and efficient technique for culturing and separating retinal stem/progenitor cells from iris tissue. Immunohistochemical staining of adult agouti mouse iris tissue revealed the presence of nestin/low-affinity neurotrophin receptor p75 (p75(NTR))-positive cells on the endothelium camerae anterioris side. Cultured mouse iris-derived cells contained little or no melanin and were found to be positive for nestin. Most nestin-positive cells were analyzed for the coexpression of p75(NTR) as a cell membrane protein. When the p75(NTR) was used as a marker to sort the cells, we obtained a dense population of nestin-positive cells. Furthermore, the nestin/p75(NTR)-positive cells were able to differentiate into neural retina cells. Thus, this culture and separation technique is useful for obtaining retinal stem/progenitor cells from adult mouse iris tissue and for the efficient production of neural retina cells.Entities:
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Year: 2010 PMID： 20857262 DOI： 10.1007/s00795-009-0486-3
Source DB: PubMed Journal: Med Mol Morphol ISSN： 1860-1499 Impact factor: 2.309