| Literature DB >> 2085177 |
G Keith1, J Desgrès, G de Murcia.
Abstract
Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly(ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly(ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length. (ii) The branching points are located at regular distances of approximately 41 derivatives from each other.Entities:
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Year: 1990 PMID: 2085177 DOI: 10.1016/0003-2697(90)90224-w
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365