OBJECTIVE: To compare the endothelial integrity of radial artery grafts harvested by minimally invasive surgery and arteries harvested conventionally for coronary artery bypass surgery (CABG) in 200 participants, who were assigned to interventions by using random allocation. METHODS: An immunohistochemical procedure with monoclonal antibodies was employed to estimate CD31 antigen and endothelial nitric oxide synthase (eNOS) expressions - markers defining endothelial integrity. RESULTS: The CD31 immunostaining revealed that the endothelial cell integrity of the minimally invasive harvested arteries was preserved in 76.1±7.4% of the circumference of luminal endothelium, which was similar to results obtained in conventionally harvested grafts (77.2±9.8%; not significant). On the other hand, eNOS immunostaining indicated that the endothelial integrity of the minimally invasive harvested grafts was preserved in 75.4±10.5% while in conventionally harvested grafts it was reduced to 42.4±14.5% of the total luminal endothelium circumference (P<0.05). CONCLUSIONS: The endothelial integrity of radial artery grafts harvested by minimally invasive surgery is better preserved than in the grafts obtained by the conventional manner. This could play an important role in improving graft patency and might represent a preliminary condition of stable functioning in coronary arterial bypasses.
RCT Entities:
OBJECTIVE: To compare the endothelial integrity of radial artery grafts harvested by minimally invasive surgery and arteries harvested conventionally for coronary artery bypass surgery (CABG) in 200 participants, who were assigned to interventions by using random allocation. METHODS: An immunohistochemical procedure with monoclonal antibodies was employed to estimate CD31 antigen and endothelial nitric oxide synthase (eNOS) expressions - markers defining endothelial integrity. RESULTS: The CD31 immunostaining revealed that the endothelial cell integrity of the minimally invasive harvested arteries was preserved in 76.1±7.4% of the circumference of luminal endothelium, which was similar to results obtained in conventionally harvested grafts (77.2±9.8%; not significant). On the other hand, eNOS immunostaining indicated that the endothelial integrity of the minimally invasive harvested grafts was preserved in 75.4±10.5% while in conventionally harvested grafts it was reduced to 42.4±14.5% of the total luminal endothelium circumference (P<0.05). CONCLUSIONS: The endothelial integrity of radial artery grafts harvested by minimally invasive surgery is better preserved than in the grafts obtained by the conventional manner. This could play an important role in improving graft patency and might represent a preliminary condition of stable functioning in coronary arterial bypasses.
Authors: Mario F Gaudino; Roberto Lorusso; Lucas B Ohmes; Navneet Narula; Patrick McIntire; Antonella Gargiulo; Maria Rosaria Bucci; Jeremy Leonard; Mohamed Rahouma; Antonino Di Franco; Guo-Wei He; Leonard N Girardi; Robert F Tranbaugh; Annarita Di Lorenzo Journal: Interact Cardiovasc Thorac Surg Date: 2019-10-01
Authors: Mateusz Puslecki; Piotr Buczkowski; Michał Nowicki; Patrycja Sujka-Kordowska; Marcin Ligowski; Marcin Misterski; Sebastian Stefaniak; Marek Dąbrowski; Agata Dabrowska; Michal Bocianski; Lukasz Szarpak; Kurt Ruetzler; Marek Jemielity; Bartlomiej Perek Journal: J Thorac Dis Date: 2018-08 Impact factor: 2.895