Literature DB >> 20844219

Rapid differentiation of Mycobacterium tuberculosis and M. bovis by high-resolution melt curve analysis.

Suheir Ereqat1, Gila Kahila Bar-Gal, Abedelmajeed Nasereddin, Kifaya Azmi, Sharif E Qaddomi, Charles L Greenblatt, Mark Spigelman, Ziad Abdeen.   

Abstract

Identification and characterization of the Mycobacterium tuberculosis strains are important for clinical and therapeutic management of tuberculosis. Real-time PCR with a high-resolution melt assay was found to improve the diagnostic process. The assay includes differentiation between M. tuberculosis and Mycobacterium bovis based on one single-nucleotide polymorphism (SNP) in the narGHJI and oxyR genes and determination of M. bovis based on the region of differences 1 (RD1). This assay correctly identified the 7 tested Mycobacterium reference strains and 52 clinical samples with a sensitivity of 2 pg DNA. This assay will help in prescribing adequate treatment and monitoring disease dynamics.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20844219      PMCID： PMC3020821          DOI： 10.1128/JCM.00943-10

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  PCR identification of Mycobacterium bovis BCG.

Authors:  E A Talbot; D L Williams; R Frothingham
Journal:  J Clin Microbiol       Date:  1997-03       Impact factor: 5.948

2.  Rapid differentiation of Mycobacterium bovis and Mycobacterium tuberculosis based on a 12.7-kb fragment by a single tube multiplex-PCR.

Authors:  C S Bakshi; D H Shah; Rishendra Verma; R K Singh; Meenakshi Malik
Journal:  Vet Microbiol       Date:  2005-08-30       Impact factor: 3.293

3.  Rapid differentiation of bovine and human tubercle bacilli based on a characteristic mutation in the bovine pyrazinamidase gene.

Authors:  A Scorpio; D Collins; D Whipple; D Cave; J Bates; Y Zhang
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

4.  Analysis of the oxyR-ahpC region in isoniazid-resistant and -susceptible Mycobacterium tuberculosis complex organisms recovered from diseased humans and animals in diverse localities.

Authors:  S Sreevatsan; X Pan; Y Zhang; V Deretic; J M Musser
Journal:  Antimicrob Agents Chemother       Date:  1997-03       Impact factor: 5.191

5.  Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from Mycobacterium tuberculosis: intraspecific M. bovis pncA sequence polymorphism.

Authors:  L E Espinosa de los Monteros; J C Galán; M Gutiérrez; S Samper; J F García Marín; C Martín; L Domínguez; L de Rafael; F Baquero; E Gómez-Mampaso; J Blázquez
Journal:  J Clin Microbiol       Date:  1998-01       Impact factor: 5.948

6.  Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis.

Authors:  S Sreevatsan; P Escalante; X Pan; D A Gillies; S Siddiqui; C N Khalaf; B N Kreiswirth; P Bifani; L G Adams; T Ficht; V S Perumaalla; M D Cave; J D van Embden; J M Musser
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

7.  A multiplex-PCR for the differentiation of Mycobacterium bovis and Mycobacterium tuberculosis.

Authors:  D H Shah; Rishendra Verma; C S Bakshi; R K Singh
Journal:  FEMS Microbiol Lett       Date:  2002-08-27       Impact factor: 2.742

8.  Multiplex real-time PCR assay for rapid identification of Mycobacterium tuberculosis complex members to the species level.

Authors:  Benjamin A Pinsky; Niaz Banaei
Journal:  J Clin Microbiol       Date:  2008-05-28       Impact factor: 5.948

9.  Rapid identification of multidrug-resistant Mycobacterium tuberculosis isolates by rpoB gene scanning using high-resolution melting curve PCR analysis.

Authors:  Ariane T Pietzka; Alexander Indra; Anna Stöger; Josef Zeinzinger; Miriam Konrad; Petra Hasenberger; Franz Allerberger; Werner Ruppitsch
Journal:  J Antimicrob Chemother       Date:  2009-04-15       Impact factor: 5.790

10.  Tokyo-172 BCG vaccination complications, Taiwan.

Authors:  Ruwen Jou; Wei Lun Huang; Wei Ju Su
Journal:  Emerg Infect Dis       Date:  2009-09       Impact factor: 6.883
View more
  7 in total

1.  Culture and molecular method for detection of Mycobacterium tuberculosis complex and Mycobacterium avium subsp. paratuberculosis in milk and dairy products.

Authors:  U Messelhäusser; P Kämpf; S Hörmansdorfer; B Wagner; B Schalch; U Busch; C Höller; P Wallner; G Barth; A Rampp
Journal:  Appl Environ Microbiol       Date:  2011-11-04       Impact factor: 4.792

2.  Improving Quantitative Power in Digital PCR through Digital High-Resolution Melting.

Authors:  April Aralar; Yixu Yuan; Kevin Chen; Yunshu Geng; Daniel Ortiz Velez; Mridu Sinha; Shelley M Lawrence; Stephanie I Fraley
Journal:  J Clin Microbiol       Date:  2020-05-26       Impact factor: 5.948

3.  Genetic characterization of Mycobacterium tuberculosis in the West Bank, Palestinian Territories.

Authors:  Suheir Ereqat; Abedelmajeed Nasereddin; Kifaya Azmi; Ziad Abdeen; Charles L Greenblatt; Mark Spigelman; Nalin Rastogi; Gila Kahila Bar-Gal
Journal:  BMC Res Notes       Date:  2012-06-07

4.  A novel multiplex real-time PCR for the identification of mycobacteria associated with zoonotic tuberculosis.

Authors:  Kate Reddington; Justin O'Grady; Siobhan Dorai-Raj; Stefan Niemann; Dick van Soolingen; Thomas Barry
Journal:  PLoS One       Date:  2011-08-09       Impact factor: 3.240

5.  Development of one-tube multiplex polymerase chain reaction (PCR) for detecting Mycobacterium bovis.

Authors:  Zhang Quan; Tan Haiming; Cai Xiaoyao; Yuan Weifeng; Jia Hong; Zhu Hongfei
Journal:  J Vet Med Sci       Date:  2016-08-18       Impact factor: 1.267

6.  First-time detection of Mycobacterium bovis in livestock tissues and milk in the West Bank, Palestinian Territories.

Authors:  Suheir Ereqat; Abedelmajeed Nasereddin; Hagai Levine; Kifaya Azmi; Amer Al-Jawabreh; Charles L Greenblatt; Ziad Abdeen; Gila Kahila Bar-Gal
Journal:  PLoS Negl Trop Dis       Date:  2013-09-12

7.  Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting.

Authors:  Joas Lucas da Silva; Gabriela Guimaraes Sousa Leite; Gisele Medeiros Bastos; Beatriz Cacciacarro Lucas; Daniel Keniti Shinohara; Joice Sayuri Takinami; Marcelo Miyata; Cristina Moreno Fajardo; André Ducati Luchessi; Clarice Queico Fujimura Leite; Rosilene Fressatti Cardoso; Rosario Dominguez Crespo Hirata; Mario Hiroyuki Hirata
Journal:  Mem Inst Oswaldo Cruz       Date:  2013-02       Impact factor: 2.743
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.