Literature DB >> 20835460

Quantitative detection of conformational transitions in a calcium sensor protein by surface plasmon resonance.

Daniele Dell'Orco1, Michael Müller, Karl-Wilhelm Koch.   

Abstract

We determined the conditions under which surface plasmon resonance can be used to monitor at real-time the Ca(2+)-induced conformational transitions of the sensor protein recoverin immobilized over a sensor chip. The equilibrium and the kinetics of conformational transitions were detected and quantified over a physiological range of Ca(2+) and protein concentrations similar to those found within cells. Structural analysis suggests that the detection principle reflects changes in the hydrodynamic properties of the protein and is not due to a mass effect. The phenomenon appears to be related to changes in the refractive index at the metal/dielectric interface.

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Year:  2010        PMID： 20835460     DOI： 10.1039/c0cc02086a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  11 in total

1.  Origin and prediction of free-solution interaction studies performed label-free.

Authors:  Darryl J Bornhop; Michael N Kammer; Amanda Kussrow; Robert A Flowers; Jens Meiler
Journal:  Proc Natl Acad Sci U S A       Date:  2016-03-09       Impact factor: 11.205

2.  Quantitative Determination of Ca2+-binding to Ca2+-sensor Proteins by Isothermal Titration Calorimetry.

Authors:  Seher Abbas; Karl-Wilhelm Koch
Journal:  Bio Protoc       Date:  2020-04-05

3.  Direct evidence for RNA-RNA interactions at the 3' end of the Hepatitis C virus genome using surface plasmon resonance.

Authors:  William Palau; Cyril Masante; Michel Ventura; Carmelo Di Primo
Journal:  RNA       Date:  2013-05-07       Impact factor: 4.942

4.  Retina specific GCAPs in zebrafish acquire functional selectivity in Ca2+-sensing by myristoylation and Mg2+-binding.

Authors:  Stefan Sulmann; Farina Vocke; Alexander Scholten; Karl-Wilhelm Koch
Journal:  Sci Rep       Date:  2015-06-10       Impact factor: 4.379

5.  Exploring the rate-limiting steps in visual phototransduction recovery by bottom-up kinetic modeling.

Authors:  Brandon M Invergo; Ludovica Montanucci; Karl-Wilhelm Koch; Jaume Bertranpetit; Daniele Dell'orco
Journal:  Cell Commun Signal       Date:  2013-05-21       Impact factor: 5.712

6.  Luminescent and paramagnetic properties of nanoparticles shed light on their interactions with proteins.

Authors:  Giuditta Dal Cortivo; Gabriel E Wagner; Paolo Cortelletti; Krishna Mohan Padmanabha Das; Klaus Zangger; Adolfo Speghini; Daniele Dell'Orco; N Helge Meyer
Journal:  Sci Rep       Date:  2018-02-21       Impact factor: 4.379

7.  Zebrafish Recoverin Isoforms Display Differences in Calcium Switch Mechanisms.

Authors:  Dana Elbers; Alexander Scholten; Karl-Wilhelm Koch
Journal:  Front Mol Neurosci       Date:  2018-09-28       Impact factor: 5.639

8.  Oligomeric state, hydrodynamic properties and target recognition of human Calcium and Integrin Binding protein 2 (CIB2).

Authors:  Giuditta Dal Cortivo; Valerio Marino; Claudio Iacobucci; Rosario Vallone; Christian Arlt; Anne Rehkamp; Andrea Sinz; Daniele Dell'Orco
Journal:  Sci Rep       Date:  2019-10-21       Impact factor: 4.379

9.  Bringing the Ca2+ sensitivity of myristoylated recoverin into the physiological range.

Authors:  Valerio Marino; Matteo Riva; Davide Zamboni; Karl-Wilhelm Koch; Daniele Dell'Orco
Journal:  Open Biol       Date:  2021-01-06       Impact factor: 6.411

10.  Effects of Membrane and Biological Target on the Structural and Allosteric Properties of Recoverin: A Computational Approach.

Authors:  Alberto Borsatto; Valerio Marino; Gianfranco Abrusci; Gianluca Lattanzi; Daniele Dell'Orco
Journal:  Int J Mol Sci       Date:  2019-10-10       Impact factor: 5.923
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