Literature DB >> 20834167

X-ray crystal structure of the DNA-binding domain of response regulator WalR essential to the cell viability of staphylococcus aureus and interaction with target DNA.

Akihiro Doi1, Toshihide Okajima, Yasuhiro Gotoh, Katsuyuki Tanizawa, Ryutaro Utsumi.   

Abstract

A bacterial two-component signal transduction system, WalK/WalR, is essential to the cell viability of Gram-positive bacteria and is therefore a potential target for the development of a new class of antibiotics. We have solved the X-ray crystal structure of the DNA-binding domain of the response regulator WalR (WalRc) from a Gram-positive pathogen Staphylococcus aureus, currently causing serious problems in public health through the acquisition of multi-drug resistance. The structure contains a winged helix-turn-helix motif and closely resembles those of WalRs of Bacillus subtilis and Enterococcus faecalis, and also that of PhoB of Escherichia coli. Gel mobility shift assays with mutant WalRs revealed specific interactions of WalR with the target DNA, as elaborated by in silico modeling of the WalRc-DNA complex.

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Year:  2010        PMID： 20834167     DOI： 10.1271/bbb.100307

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  6 in total

1.  Involvement of WalK (VicK) phosphatase activity in setting WalR (VicR) response regulator phosphorylation level and limiting cross-talk in Streptococcus pneumoniae D39 cells.

Authors:  Kyle J Wayne; Shuo Li; Krystyna M Kazmierczak; Ho-Ching T Tsui; Malcolm E Winkler
Journal:  Mol Microbiol       Date:  2012-09-27       Impact factor: 3.501

2.  Isolation and characterization of signermycin B, an antibiotic that targets the dimerization domain of histidine kinase WalK.

Authors:  Takafumi Watanabe; Masayuki Igarashi; Toshihide Okajima; Eiji Ishii; Hirokazu Kino; Masaki Hatano; Ryuichi Sawa; Maya Umekita; Tomoyuki Kimura; Sho Okamoto; Yoko Eguchi; Yuzuru Akamatsu; Ryutaro Utsumi
Journal:  Antimicrob Agents Chemother       Date:  2012-04-23       Impact factor: 5.191

3.  Structure, bioactivity, and resistance mechanism of streptomonomicin, an unusual lasso Peptide from an understudied halophilic actinomycete.

Authors:  Mikhail Metelev; Jonathan I Tietz; Joel O Melby; Patricia M Blair; Lingyang Zhu; Itamar Livnat; Konstantin Severinov; Douglas A Mitchell
Journal:  Chem Biol       Date:  2015-01-15

4.  Distinct single amino acid replacements in the control of virulence regulator protein differentially impact streptococcal pathogenesis.

Authors:  Nicola Horstmann; Pranoti Sahasrabhojane; Bryce Suber; Muthiah Kumaraswami; Randall J Olsen; Anthony Flores; James M Musser; Richard G Brennan; Samuel A Shelburne
Journal:  PLoS Pathog       Date:  2011-10-20       Impact factor: 6.823

5.  Evolution of multidrug resistance during Staphylococcus aureus infection involves mutation of the essential two component regulator WalKR.

Authors:  Benjamin P Howden; Christopher R E McEvoy; David L Allen; Kyra Chua; Wei Gao; Paul F Harrison; Jan Bell; Geoffrey Coombs; Vicki Bennett-Wood; Jessica L Porter; Roy Robins-Browne; John K Davies; Torsten Seemann; Timothy P Stinear
Journal:  PLoS Pathog       Date:  2011-11-10       Impact factor: 6.823

6.  Structure and dynamics of polymyxin-resistance-associated response regulator PmrA in complex with promoter DNA.

Authors:  Yuan-Chao Lou; Tsai-Hsuan Weng; Yi-Chuan Li; Yi-Fen Kao; Wei-Feng Lin; Hwei-Ling Peng; Shan-Ho Chou; Chwan-Deng Hsiao; Chinpan Chen
Journal:  Nat Commun       Date:  2015-11-13       Impact factor: 14.919
  6 in total

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