BACKGROUND: Increased cell-free DNA (cf-DNA) and the integrity of cf-DNA in plasma of patients with cancer has been described. We investigated the clinical utility of cf-DNA in the detection and monitoring of progression of leukemia. METHODS: Plasma samples from 60 patients with acute leukemia were analyzed in comparison to plasma from 30 healthy controls. Plasma DNA was determined by quantitative PCR (qPCR) by amplifying the β-actin gene (ACTB). The DNA integrity index was calculated as the ratio of qPCR results (ACTB384/106). Paired diagnostic/complete remission (CR)/relapse samples from eight of 60 patients were analyzed, and the minimum residual disease (MRD) situations were monitored. RESULTS: DNA concentrations (median: 8.80 ng/mL, p=0.004) and DNA integrity (median: 0.51, p<0.001) in cancer patients were significantly higher. Receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve of DNA and DNA integrity were 0.79 and 0.88, respectively. DNA integrity at CR had a distinct reduction and then an increase at relapse. DNA integrity in CR cases was higher than that observed in healthy controls. CONCLUSIONS: Our preliminary data suggest that plasma DNA integrity is increased in acute leukemia and may be a potential biomarker for monitoring MRD. However, more work is needed.
BACKGROUND: Increased cell-free DNA (cf-DNA) and the integrity of cf-DNA in plasma of patients with cancer has been described. We investigated the clinical utility of cf-DNA in the detection and monitoring of progression of leukemia. METHODS: Plasma samples from 60 patients with acute leukemia were analyzed in comparison to plasma from 30 healthy controls. Plasma DNA was determined by quantitative PCR (qPCR) by amplifying the β-actin gene (ACTB). The DNA integrity index was calculated as the ratio of qPCR results (ACTB384/106). Paired diagnostic/complete remission (CR)/relapse samples from eight of 60 patients were analyzed, and the minimum residual disease (MRD) situations were monitored. RESULTS: DNA concentrations (median: 8.80 ng/mL, p=0.004) and DNA integrity (median: 0.51, p<0.001) in cancerpatients were significantly higher. Receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve of DNA and DNA integrity were 0.79 and 0.88, respectively. DNA integrity at CR had a distinct reduction and then an increase at relapse. DNA integrity in CR cases was higher than that observed in healthy controls. CONCLUSIONS: Our preliminary data suggest that plasma DNA integrity is increased in acute leukemia and may be a potential biomarker for monitoring MRD. However, more work is needed.
Authors: Peiyong Jiang; Carol W M Chan; K C Allen Chan; Suk Hang Cheng; John Wong; Vincent Wai-Sun Wong; Grace L H Wong; Stephen L Chan; Tony S K Mok; Henry L Y Chan; Paul B S Lai; Rossa W K Chiu; Y M Dennis Lo Journal: Proc Natl Acad Sci U S A Date: 2015-02-02 Impact factor: 11.205
Authors: Pâmela Oliveira Delgado; Beatriz Costa A Alves; Flávia de Sousa Gehrke; Renata Kelly Kuniyoshi; Marcelo Langer Wroclavski; Auro Del Giglio; Fernando Luiz Affonso Fonseca Journal: Tumour Biol Date: 2012-12-27
Authors: Suk Hang Cheng; Kin Mang Lau; Chi Kong Li; Natalie P H Chan; Rosalina K L Ip; Chi Keung Cheng; Vincent Lee; Matthew M K Shing; Alex W K Leung; Shau Yin Ha; Daniel K L Cheuk; Anselm C W Lee; Chak Ho Li; Chung Wing Luk; Siu Cheung Ling; Ondrej Hrusak; Ester Mejstrikova; Yonna Leung; Margaret H L Ng Journal: PLoS One Date: 2013-07-25 Impact factor: 3.240