AIM: To produce two-dimensional reconstruction maps of the subepithelial nerve plexus (SEP) in living cornea by in vivo laser scanning confocal microscopy in real time. METHODS: In vivo confocal laser scanning microscopy (Heidelberg Retinal Tomograph II in conjunction with the Rostock Cornea Module) was performed on normal eyes (n=6) and eyes after laser-assisted in situ keratomileusis (LASIK) (n=4). Source data (frame rate 30 Hz) were used to create large-scale maps of the scanned area in Automatic Real Time composite mode. The algorithm aligns single live images onto the previously mapped composite image using landmark feature-based image processing. RESULTS: Real-time mapping of the SEP was performed on a large-scale area up to 3.2x3.2 mm (3072x3072 pixels) in healthy subjects and in post-LASIK patients. Two-dimensional structures of the SEP were imaged in all 10 eyes. Mapping quality as well as acquisition time were dependent on subject compliance and examiner experience. CONCLUSION: The described method permits real-time in vivo mapping of the SEP, thus providing the necessary basis for statistically robust conclusions concerning morphometric plexus alterations.
AIM: To produce two-dimensional reconstruction maps of the subepithelial nerve plexus (SEP) in living cornea by in vivo laser scanning confocal microscopy in real time. METHODS: In vivo confocal laser scanning microscopy (Heidelberg Retinal Tomograph II in conjunction with the Rostock Cornea Module) was performed on normal eyes (n=6) and eyes after laser-assisted in situ keratomileusis (LASIK) (n=4). Source data (frame rate 30 Hz) were used to create large-scale maps of the scanned area in Automatic Real Time composite mode. The algorithm aligns single live images onto the previously mapped composite image using landmark feature-based image processing. RESULTS: Real-time mapping of the SEP was performed on a large-scale area up to 3.2x3.2 mm (3072x3072 pixels) in healthy subjects and in post-LASIK patients. Two-dimensional structures of the SEP were imaged in all 10 eyes. Mapping quality as well as acquisition time were dependent on subject compliance and examiner experience. CONCLUSION: The described method permits real-time in vivo mapping of the SEP, thus providing the necessary basis for statistically robust conclusions concerning morphometric plexus alterations.
Authors: B Köhler; S Allgeier; A Bartschat; R F Guthoff; S Bohn; K-M Reichert; O Stachs; K Winter; R Mikut Journal: Ophthalmologe Date: 2017-07 Impact factor: 1.059
Authors: Ahmad Kheirkhah; Rodrigo Muller; Janine Mikolajczak; Ai Ren; Ella Maria Kadas; Hanna Zimmermann; Harald Pruess; Friedemann Paul; Alexander U Brandt; Pedram Hamrah Journal: Invest Ophthalmol Vis Sci Date: 2015-09 Impact factor: 4.799
Authors: Michael A Whitney; Jessica L Crisp; Linda T Nguyen; Beth Friedman; Larry A Gross; Paul Steinbach; Roger Y Tsien; Quyen T Nguyen Journal: Nat Biotechnol Date: 2011-02-06 Impact factor: 54.908