PURPOSE: To investigate the relationship between chromosomal radiosensitivity and early-onset cancer under the age of 35 years and to examine the heritability of chromosomal radiosensitivity. MATERIALS AND METHODS: Peripheral blood lymphocytes were cultured for 72 hours prior to being irradiated with 0.5 Gy, 300 kV X-rays. Colcemid was added to cultures 30 min post-irradiation. Cultures were harvested 90 min post-irradiation and analysed for chromatid gaps and breaks. Heritability was estimated using Sequential Oligogenic Linkage Analysis Routines (SOLAR) software and by segregation analysis. RESULTS: Elevated radiosensitivity was seen for seven out of 29 (24.1%) cancer survivors, three out of 29 (10.3%) partners and 10 out of 53 (20.8%) offspring. Although the proportion of individuals displaying enhanced radiosensitivity was twice as high in both the cancer survivor and offspring groups than the partner controls, neither reached statistical significance. Heritability analysis of the radiosensitive phenotype suggested 57.9-78.0% of the variance could be attributed to genetic factors. CONCLUSION: An association between G(2) chromosomal radiosensitivity and childhood and young adult cancer is suggested but was not statistically significant. In contrast, there is strong evidence for heritability of the radiosensitive phenotype. The cancer survivors included a broad range of malignancies and future studies should focus on specific cancers with known or likely faults in deoxyribonucleic acid (DNA) damage recognition and repair mechanisms.
PURPOSE: To investigate the relationship between chromosomal radiosensitivity and early-onset cancer under the age of 35 years and to examine the heritability of chromosomal radiosensitivity. MATERIALS AND METHODS: Peripheral blood lymphocytes were cultured for 72 hours prior to being irradiated with 0.5 Gy, 300 kV X-rays. Colcemid was added to cultures 30 min post-irradiation. Cultures were harvested 90 min post-irradiation and analysed for chromatid gaps and breaks. Heritability was estimated using Sequential Oligogenic Linkage Analysis Routines (SOLAR) software and by segregation analysis. RESULTS: Elevated radiosensitivity was seen for seven out of 29 (24.1%) cancer survivors, three out of 29 (10.3%) partners and 10 out of 53 (20.8%) offspring. Although the proportion of individuals displaying enhanced radiosensitivity was twice as high in both the cancer survivor and offspring groups than the partner controls, neither reached statistical significance. Heritability analysis of the radiosensitive phenotype suggested 57.9-78.0% of the variance could be attributed to genetic factors. CONCLUSION: An association between G(2) chromosomal radiosensitivity and childhood and young adult cancer is suggested but was not statistically significant. In contrast, there is strong evidence for heritability of the radiosensitive phenotype. The cancer survivors included a broad range of malignancies and future studies should focus on specific cancers with known or likely faults in deoxyribonucleic acid (DNA) damage recognition and repair mechanisms.
Authors: G B Curwen; J F Winther; E J Tawn; V Smart; C A Whitehouse; G S Rees; J H Olsen; P Guldberg; C Rechnitzer; H Schrøder; P E Bryant; X Sheng; H S Lee; R Chakraborty; J D Boice Journal: Br J Cancer Date: 2005-10-31 Impact factor: 7.640
Authors: Yan Guo; Qiuyin Cai; David C Samuels; Fei Ye; Jirong Long; Chung-I Li; Jeanette F Winther; E Janet Tawn; Marilyn Stovall; Päivi Lähteenmäki; Nea Malila; Shawn Levy; Christian Shaffer; Yu Shyr; Xiao-Ou Shu; John D Boice Journal: Mutat Res Date: 2012-02-24 Impact factor: 2.433
Authors: Christian Garm; Maria Moreno-Villanueva; Alexander Bürkle; Lisbeth Aagaard Larsen; Vilhelm A Bohr; Kaare Christensen; Tinna Stevnsner Journal: Environ Mol Mutagen Date: 2013-06-25 Impact factor: 3.216
Authors: Gillian B Curwen; Samantha Murphy; Elizabeth J Tawn; Jeanette F Winther; John D Boice Journal: Environ Mol Mutagen Date: 2011-01 Impact factor: 3.216
Authors: C J Talbot; G A Tanteles; G C Barnett; N G Burnet; J Chang-Claude; C E Coles; S Davidson; A M Dunning; J Mills; R J S Murray; O Popanda; P Seibold; C M L West; J R Yarnold; R P Symonds Journal: Br J Cancer Date: 2012-07-05 Impact factor: 7.640
Authors: Ian P M Tomlinson; Richard S Houlston; Grant W Montgomery; Oliver M Sieber; Malcolm G Dunlop Journal: Mutagenesis Date: 2012-03 Impact factor: 3.000