Chromatographic and electrophoretic techniques used in the analysis of triazole antifungal agents-a review.

Systematic review of literature coupled with integrative research of published data for triazole antifungal agents was done. The investigated literature covered chromatographic and electrophoretic methods developed in the last 10 years (2000-2009). The aim of this review was to compare different methodologies, assess preferences in the selection of analytical methods and to find still existing analytical problems. Last decade is characterized by dynamic development of instrumental methods, that results in advance and diversity of applied analytical procedures. The main focus was given to high-performance liquid chromatography (HPLC), the technique of choice in the analysis of most of pharmaceuticals. The review includes literature on 8 triazole antifungal drugs: fluconazole, itraconazole and terconazole from the first generation and posaconazole, voriconazole, ravuconazole, isavuconazole and albaconazole classified in second generation. Investigations of pharmaceutical formulations and biological samples were considered. Copyright (c) 2010 Elsevier B.V. All rights reserved.