Cristiane Luchese1, Cristina W Nogueira. 1. Departamento de Química, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, Brazil.
Abstract
OBJECTIVES: The antioxidant action of diphenyl diselenide ((PhSe)(2)) is attributed to the mechanism by which (PhSe)(2) has pharmacological activity. Although (PhSe)(2) has glutathione peroxidase mimetic activity, the exact mechanism involved in its antioxidant effect has not yet been completely elucidated. In the present study, mechanisms involved in the antioxidant property of (PhSe)(2) (1-50 microM) were investigated. METHODS: Dehydroascorbate (DHA) reductase- and glutathione S-transferase (GST)-like activity, 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity and the protection against the oxidation of Fe(2+) were evaluated. KEY FINDINGS: (PhSe)(2) at concentrations equal to, or greater than, 5 microM showed DHA reductase- and GST-like activity. (PhSe)(2) was not a scavenger of DPPH or ABTS radicals and did not protect against the oxidation of Fe(2+). CONCLUSIONS: These results clearly indicated that DHA reductase- and GST-like activity are the mechanisms involved in the antioxidant effect of (PhSe)(2).
OBJECTIVES: The antioxidant action of diphenyl diselenide ((PhSe)(2)) is attributed to the mechanism by which (PhSe)(2) has pharmacological activity. Although (PhSe)(2) has glutathione peroxidase mimetic activity, the exact mechanism involved in its antioxidant effect has not yet been completely elucidated. In the present study, mechanisms involved in the antioxidant property of (PhSe)(2) (1-50 microM) were investigated. METHODS:Dehydroascorbate (DHA) reductase- and glutathione S-transferase (GST)-like activity, 2,2'-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical-scavenging activity and the protection against the oxidation of Fe(2+) were evaluated. KEY FINDINGS: (PhSe)(2) at concentrations equal to, or greater than, 5 microM showed DHA reductase- and GST-like activity. (PhSe)(2) was not a scavenger of DPPH or ABTS radicals and did not protect against the oxidation of Fe(2+). CONCLUSIONS: These results clearly indicated that DHA reductase- and GST-like activity are the mechanisms involved in the antioxidant effect of (PhSe)(2).
Authors: César A Brüning; Marina Prigol; Cristiane Luchese; Cristiano R Jesse; Marta M M F Duarte; Silvane S Roman; Cristina W Nogueira Journal: Neurochem Res Date: 2012-07-31 Impact factor: 3.996
Authors: Charlene Menezes; Ignacio Ruiz-Jarabo; Juan Antonio Martos-Sitcha; Jossiele Leitemperger; Bernardo Baldisserotto; Juan Miguel Mancera; Denis Broock Rosemberg; Vania Lucia Loro Journal: PLoS One Date: 2014-12-03 Impact factor: 3.240