Literature DB >> 20737579

The apicomplexan Cryptosporidium parvum possesses a single mitochondrial-type ferredoxin and ferredoxin:NADP+ reductase system.

Cheng Lei1, S Dean Rider, Cai Wang, Haili Zhang, Xiangshi Tan, Guan Zhu.   

Abstract

We have successfully expressed recombinant mitochondrial-type ferredoxin (mtFd) and ferredoxin:NADP(+) reductase (mtFNR) from Cryptosporidium parvum and characterized their biochemical features for the first time for an apicomplexan. Both C. parvum mtFd (CpmtFd) and FNR (CpmtFNR) were obtained and purified as holo-proteins, in which the correct assembly of [2Fe-2S] cluster in Fd and that of FAD in FNR were confirmed and characterized by UV/vis and electron paramagnetic resonance. These proteins were fully functional and CpmtFNR was capable of transferring electrons from NADPH to CpmtFd in a cytochrome c-coupled assay that followed a typical Michaelis-Menten kinetics. Apicomplexan mtFd and mtFNR proteins were evolutionarily divergent from their counterparts in humans and animals and could be explored as potential drug targets in Cryptosporidium and other apicomplexans.

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Year:  2010        PMID： 20737579      PMCID： PMC3005779          DOI： 10.1002/pro.487

Source DB:  PubMed          Journal:  Protein Sci        ISSN： 0961-8368            Impact factor:   6.725


  40 in total

Review 1.  The apicoplast: a new member of the plastid family.

Authors:  E Maréchal; M F Cesbron-Delauw
Journal:  Trends Plant Sci       Date:  2001-05       Impact factor: 18.313

Review 2.  Eukaryotic genomes contain a [2Fez.sbnd;2S] ferredoxin isoform with a conserved C-terminal sequence motif.

Authors:  Frank Seeber
Journal:  Trends Biochem Sci       Date:  2002-11       Impact factor: 13.807

3.  Toxoplasma gondii: analysis of the active site insertion of its ferredoxin-NADP(+)-reductase by peptide-specific antibodies and homology-based modeling.

Authors:  Anika Bednarek; Sabine Wiek; Klaus Lingelbach; Frank Seeber
Journal:  Exp Parasitol       Date:  2003 Jan-Feb       Impact factor: 2.011

4.  Cryptosporidium parvum appears to lack a plastid genome.

Authors:  G Zhu; M J Marchewka; J S Keithly
Journal:  Microbiology       Date:  2000-02       Impact factor: 2.777

5.  Pyruvate : NADP+ oxidoreductase from the mitochondrion of Euglena gracilis and from the apicomplexan Cryptosporidium parvum: a biochemical relic linking pyruvate metabolism in mitochondriate and amitochondriate protists.

Authors:  C Rotte; F Stejskal; G Zhu; J S Keithly; W Martin
Journal:  Mol Biol Evol       Date:  2001-05       Impact factor: 16.240

6.  Apicomplexan parasites possess distinct nuclear-encoded, but apicoplast-localized, plant-type ferredoxin-NADP+ reductase and ferredoxin.

Authors:  M Vollmer; N Thomsen; S Wiek; F Seeber
Journal:  J Biol Chem       Date:  2000-10-30       Impact factor: 5.157

7.  Ferredoxin-NADP+ reductase and ferredoxin of the protozoan parasite Toxoplasma gondii interact productively in Vitro and in Vivo.

Authors:  Vittorio Pandini; Gianluca Caprini; Nadine Thomsen; Alessandro Aliverti; Frank Seeber; Giuliana Zanetti
Journal:  J Biol Chem       Date:  2002-10-04       Impact factor: 5.157

8.  Intron-containing beta-tubulin transcripts in Cryptosporidium parvum cultured in vitro.

Authors:  Xiaomin Cai; Cheryl A Lancto; Mitchell S Abrahamsen; Guan Zhu
Journal:  Microbiology       Date:  2004-05       Impact factor: 2.777

9.  Complete genome sequence of the apicomplexan, Cryptosporidium parvum.

Authors:  Mitchell S Abrahamsen; Thomas J Templeton; Shinichiro Enomoto; Juan E Abrahante; Guan Zhu; Cheryl A Lancto; Mingqi Deng; Chang Liu; Giovanni Widmer; Saul Tzipori; Gregory A Buck; Ping Xu; Alan T Bankier; Paul H Dear; Bernard A Konfortov; Helen F Spriggs; Lakshminarayan Iyer; Vivek Anantharaman; L Aravind; Vivek Kapur
Journal:  Science       Date:  2004-03-25       Impact factor: 47.728

Review 10.  Mitochondria in malaria and related parasites: ancient, diverse and streamlined.

Authors:  Michael W Mather; Akhil B Vaidya
Journal:  J Bioenerg Biomembr       Date:  2008-09-24       Impact factor: 3.853
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  2 in total

1.  Validation of IMP dehydrogenase inhibitors in a mouse model of cryptosporidiosis.

Authors:  Suresh Kumar Gorla; Nina N McNair; Guangyi Yang; Song Gao; Ming Hu; Venkatakrishna R Jala; Bodduluri Haribabu; Boris Striepen; Gregory D Cuny; Jan R Mead; Lizbeth Hedstrom
Journal:  Antimicrob Agents Chemother       Date:  2013-12-23       Impact factor: 5.191

2.  Identification of Sequences Encoding Symbiodinium minutum Mitochondrial Proteins.

Authors:  Erin R Butterfield; Christopher J Howe; R Ellen R Nisbet
Journal:  Genome Biol Evol       Date:  2016-01-21       Impact factor: 3.416
  2 in total

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