Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Isolation of the 5'-end of plant genes from genomic DNA by TATA-box-based degenerate primers.

Literature DB >> 20730510

Isolation of the 5'-end of plant genes from genomic DNA by TATA-box-based degenerate primers.

Yanwu Guo¹, Lanqing Ma, Yunpeng Ji, Gaobin Pu, Benye Liu, Zhigao Du, Guofeng Li, Hechun Ye, Hong Wang.

Abstract

We report a rapid and simple method for isolating the 5'-end of plant genes from genomic DNA by polymerase chain reaction (PCR) with TATA-box-based degenerate primers (TDPs). The TDPs were specially designed according to the TATA box, which is conserved in the promoter region of most plant genes. The unknown 5'-ends of several genes in different plants were isolated by PCR with gene-specific primers of the known core fragment and the TDPs. Our method does not require the arduous RNA manipulations and expensive enzyme treatments of the popular rapid amplification of cDNA ends (RACE) and its variants, and so could be a cheap practical alternative.

Mesh：

Substances：

Year: 2011 PMID： 20730510 DOI： 10.1007/s12033-010-9323-0

Source DB: PubMed Journal: Mol Biotechnol ISSN： 1073-6085 Impact factor: 2.695

19 in total

1. A simple and reliable 5'-RACE approach.

Authors: G Schramm; I Bruchhaus; T Roeder
Journal: Nucleic Acids Res Date: 2000-11-15 Impact factor: 16.971

2. cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.

Authors: Z Ye; J R Connor
Journal: Biochem Biophys Res Commun Date: 2000-08-18 Impact factor: 3.575

3. PlantProm: a database of plant promoter sequences.

Authors: Ilham A Shahmuradov; Alex J Gammerman; John M Hancock; Peter M Bramley; Victor V Solovyev
Journal: Nucleic Acids Res Date: 2003-01-01 Impact factor: 16.971

4. Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

Authors: A B Troutt; M G McHeyzer-Williams; B Pulendran; G J Nossal
Journal: Proc Natl Acad Sci U S A Date: 1992-10-15 Impact factor: 11.205

5. An efficient and economic enhancer mix for PCR.

Authors: Markus Ralser; Robert Querfurth; Hans-Jörg Warnatz; Hans Lehrach; Marie-Laure Yaspo; Sylvia Krobitsch
Journal: Biochem Biophys Res Commun Date: 2006-07-05 Impact factor: 3.575

6. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking.

Authors: Y G Liu; R F Whittier
Journal: Genomics Date: 1995-02-10 Impact factor: 5.736

Isolation of the 5'-end of plant genes from genomic DNA by TATA-box-based degenerate primers.

1. A simple and reliable 5'-RACE approach.

2. cDNA cloning by amplification of circularized first strand cDNAs reveals non-IRE-regulated iron-responsive mRNAs.

3. PlantProm: a database of plant promoter sequences.

4. Ligation-anchored PCR: a simple amplification technique with single-sided specificity.

5. An efficient and economic enhancer mix for PCR.

6. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking.

7. A highly sensitive method for mapping the 5' termini of mRNAs.

8. Inverse polymerase chain reaction. An efficient approach to cloning cDNA ends.

9. A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide.

10. A novel type III polyketide synthase encoded by a three-intron gene from Polygonum cuspidatum.

1. Octaketide Synthase from Polygonum cuspidatum Implements Emodin Biosynthesis in Arabidopsis thaliana.