Literature DB >> 20729858

The breast cancer tumor suppressor BRCA2 promotes the specific targeting of RAD51 to single-stranded DNA.

Tina Thorslund1, Michael J McIlwraith, Sarah A Compton, Sergey Lekomtsev, Mark Petronczki, Jack D Griffith, Stephen C West.   

Abstract

Individuals with BRCA2 mutations are predisposed to breast cancers owing to genome instability. To determine the functions of BRCA2, the human protein was purified. It was found to bind selectively to single-stranded DNA (ssDNA), and to ssDNA in tailed duplexes and replication fork structures. Monomeric and dimeric forms of BRCA2 were observed by EM. BRCA2 directed the binding of RAD51 recombinase to ssDNA, reduced the binding of RAD51 to duplex DNA and stimulated RAD51-mediated DNA strand exchange. These observations provide a molecular basis for the role of BRCA2 in the maintenance of genome stability.

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Year:  2010        PMID： 20729858      PMCID： PMC4041013          DOI： 10.1038/nsmb.1905

Source DB:  PubMed          Journal:  Nat Struct Mol Biol        ISSN： 1545-9985            Impact factor:   15.369


  22 in total

1.  Evidence that the tumor-suppressor protein BRCA2 does not regulate cytokinesis in human cells.

Authors:  Sergey Lekomtsev; Julien Guizetti; Andrei Pozniakovsky; Daniel W Gerlich; Mark Petronczki
Journal:  J Cell Sci       Date:  2010-03-31       Impact factor: 5.285

2.  Dss1 interaction with Brh2 as a regulatory mechanism for recombinational repair.

Authors:  Qingwen Zhou; Milorad Kojic; Zhimin Cao; Michael Lisby; Nayef A Mazloum; William K Holloman
Journal:  Mol Cell Biol       Date:  2007-01-29       Impact factor: 4.272

3.  Inactivation of RAD52 aggravates RAD54 defects in mice but not in Schizosaccharomyces pombe.

Authors:  Femke A T de Vries; José B M Zonneveld; Annemarie van Duijn-Goedhart; Marianne Roodbergen; Jan Boei; Paul P W van Buul; Jeroen Essers; Harry van Steeg; Albert A van Zeeland; Jan van Benthem; Albert Pastink
Journal:  DNA Repair (Amst)       Date:  2005-09-28

Review 4.  Assembly of RecA-like recombinases: distinct roles for mediator proteins in mitosis and meiosis.

Authors:  S L Gasior; H Olivares; U Ear; D M Hari; R Weichselbaum; D K Bishop
Journal:  Proc Natl Acad Sci U S A       Date:  2001-07-17       Impact factor: 11.205

5.  CeBRC-2 stimulates D-loop formation by RAD-51 and promotes DNA single-strand annealing.

Authors:  Mark I R Petalcorin; Jane Sandall; Dale B Wigley; Simon J Boulton
Journal:  J Mol Biol       Date:  2006-06-27       Impact factor: 5.469

6.  The BRC repeats of human BRCA2 differentially regulate RAD51 binding on single- versus double-stranded DNA to stimulate strand exchange.

Authors:  Mahmud K K Shivji; Shreyas R Mukund; Eeson Rajendra; Shaoxia Chen; Judith M Short; Jane Savill; David Klenerman; Ashok R Venkitaraman
Journal:  Proc Natl Acad Sci U S A       Date:  2009-07-23       Impact factor: 11.205

7.  Targeted inactivation of mouse RAD52 reduces homologous recombination but not resistance to ionizing radiation.

Authors:  T Rijkers; J Van Den Ouweland; B Morolli; A G Rolink; W M Baarends; P P Van Sloun; P H Lohman; A Pastink
Journal:  Mol Cell Biol       Date:  1998-11       Impact factor: 4.272

8.  Cancer-associated mutations in BRC domains of BRCA2 affect homologous recombination induced by Rad51.

Authors:  Asaf Tal; Rinat Arbel-Goren; Joel Stavans
Journal:  J Mol Biol       Date:  2009-09-10       Impact factor: 5.469

9.  The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51.

Authors:  Aura Carreira; Jovencio Hilario; Ichiro Amitani; Ronald J Baskin; Mahmud K K Shivji; Ashok R Venkitaraman; Stephen C Kowalczykowski
Journal:  Cell       Date:  2009-03-20       Impact factor: 41.582

10.  A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchange.

Authors:  Mahmud K K Shivji; Owen R Davies; Jane M Savill; Debbie L Bates; Luca Pellegrini; Ashok R Venkitaraman
Journal:  Nucleic Acids Res       Date:  2006-08-16       Impact factor: 16.971
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  130 in total

1.  DNA repair: A protein giant in its entirety.

Authors:  Lee Zou
Journal:  Nature       Date:  2010-10-07       Impact factor: 49.962

2.  A variant of the breast cancer type 2 susceptibility protein (BRC) repeat is essential for the RECQL5 helicase to interact with RAD51 recombinase for genome stabilization.

Authors:  M Nurul Islam; Nicolas Paquet; David Fox; Eloise Dray; Xiao-Feng Zheng; Hannah Klein; Patrick Sung; Weidong Wang
Journal:  J Biol Chem       Date:  2012-05-29       Impact factor: 5.157

3.  Small-molecule inhibitors identify the RAD52-ssDNA interaction as critical for recovery from replication stress and for survival of BRCA2 deficient cells.

Authors:  Sarah R Hengel; Eva Malacaria; Laura Folly da Silva Constantino; Fletcher E Bain; Andrea Diaz; Brandon G Koch; Liping Yu; Meng Wu; Pietro Pichierri; M Ashley Spies; Maria Spies
Journal:  Elife       Date:  2016-07-19       Impact factor: 8.140

4.  Coupling of Homologous Recombination and the Checkpoint by ATR.

Authors:  Rémi Buisson; Joshi Niraj; Amélie Rodrigue; Chu Kwen Ho; Johannes Kreuzer; Tzeh Keong Foo; Emilie J-L Hardy; Graham Dellaire; Wilhelm Haas; Bing Xia; Jean-Yves Masson; Lee Zou
Journal:  Mol Cell       Date:  2017-01-12       Impact factor: 17.970

Review 5.  Homologous recombination and human health: the roles of BRCA1, BRCA2, and associated proteins.

Authors:  Rohit Prakash; Yu Zhang; Weiran Feng; Maria Jasin
Journal:  Cold Spring Harb Perspect Biol       Date:  2015-04-01       Impact factor: 10.005

6.  Many ways to loop DNA.

Authors:  Jack D Griffith
Journal:  J Biol Chem       Date:  2013-09-04       Impact factor: 5.157

7.  GST-His purification: a two-step affinity purification protocol yielding full-length purified proteins.

Authors:  Ranjan Maity; Joris Pauty; Jana Krietsch; Rémi Buisson; Marie-Michelle Genois; Jean-Yves Masson
Journal:  J Vis Exp       Date:  2013-10-29       Impact factor: 1.355

8.  USP1 Is Required for Replication Fork Protection in BRCA1-Deficient Tumors.

Authors:  Kah Suan Lim; Heng Li; Emma A Roberts; Emily F Gaudiano; Connor Clairmont; Larissa Alina Sambel; Karthikeyan Ponnienselvan; Jessica C Liu; Chunyu Yang; David Kozono; Kalindi Parmar; Timur Yusufzai; Ning Zheng; Alan D D'Andrea
Journal:  Mol Cell       Date:  2018-12-20       Impact factor: 17.970

Review 9.  Functional assays for analysis of variants of uncertain significance in BRCA2.

Authors:  Lucia Guidugli; Aura Carreira; Sandrine M Caputo; Asa Ehlen; Alvaro Galli; Alvaro N A Monteiro; Susan L Neuhausen; Thomas V O Hansen; Fergus J Couch; Maaike P G Vreeswijk
Journal:  Hum Mutat       Date:  2013-12-03       Impact factor: 4.878

10.  Nascent DNA synthesis during homologous recombination is synergistically promoted by the rad51 recombinase and DNA homology.

Authors:  Maureen M Mundia; Vatsal Desai; Alissa C Magwood; Mark D Baker
Journal:  Genetics       Date:  2014-02-28       Impact factor: 4.562
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