Literature DB >> 207287

Analysis of DNA structure by hydroxyapatite columns and ethidium bromide fluorescence techniques. A comparative study and effect on DNA binding.

P Davis, M Burrington, A S Russell, A R Morgan.   

Abstract

Seven duplex DNA preparations have been structurally analyzed by hydroxyapatite column chromatography and an ethidium bromide fluorescence technique. Significant contamination of one preparation with single-stranded DNA was detected by hydroxyapatite column chromatography. Five of the other six preparations were found to contain significant single-stranded regions by the ethidium bromide fluorescence technique. Synthetic poly dAT was found to be duplex in structure. The presence of single-stranded regions considerably influenced DNA binding results in a radioimmunoassay.

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Year:  1978        PMID： 207287     DOI： 10.1002/art.1780210402

Source DB:  PubMed          Journal:  Arthritis Rheum        ISSN： 0004-3591


  8 in total

1.  Structural requirements of DNA used in the Farr assay to detect antibodies directed against double-stranded DNA.

Authors:  K M Pollard; J Webb
Journal:  Rheumatol Int       Date:  1987       Impact factor: 2.631

2.  Efficient generation in vitro, from human peripheral blood cells, of monoclonal Epstein-Barr virus transformants producing specific antibody to a variety of antigens without prior deliberate immunization.

Authors:  L Winger; C Winger; P Shastry; A Russell; M Longenecker
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205

3.  Measurement of serum DNA binding in chronic active hepatitis and systemic lupus erythematosus using the Farr assay.

Authors:  K M Pollard; R Steele; S Hogg; J Webb
Journal:  Rheumatol Int       Date:  1986       Impact factor: 2.631

4.  A simple, rapid ELISA method for the detection of DNA antibodies.

Authors:  R P Stokes; A Cordwell; R A Thompson
Journal:  J Clin Pathol       Date:  1982-05       Impact factor: 3.411

5.  Neutropenia in rheumatoid arthritis: studies on possible contributing factors.

Authors:  R C Bucknall; P Davis; P A Bacon; J V Jones
Journal:  Ann Rheum Dis       Date:  1982-06       Impact factor: 19.103

6.  Seronegative chronic polyarthritis: clinical and serological correlates.

Authors:  J L Rasker; P Davis; P A Bacon
Journal:  Ann Rheum Dis       Date:  1980-12       Impact factor: 19.103

7.  Antibody to synthetic poly dAT: correlation with antibody to native DNA and specificity for SLE.

Authors:  P Davis; D Makinen
Journal:  J Clin Pathol       Date:  1980-07       Impact factor: 3.411

8.  Immunochemical characteristics of antibodies to DNA in patients with active systemic lupus erythematosus.

Authors:  S P Ballou; I Kushner
Journal:  Clin Exp Immunol       Date:  1979-07       Impact factor: 4.330
  8 in total

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