An environmental enrichment model for mice.

Environmental enrichment for animals is a combination of complex inanimate and social stimulation and generally consists of housing conditions that facilitate enhanced sensory, cognitive, motor and social stimulation relative to standard housing conditions. One of the most robust effects of environmental enrichment is the reduction of anxiety levels. However, the extreme variability in enrichment protocols may account for some of the inconsistencies in its effects and the variance among results reported by different laboratories. In this protocol, we describe a simple environmental enrichment strategy for the induction of a robust and replicable anxiolytic-like effect in mice. We provide detailed instructions on how to build an enrichment cage that is specially designed for easy manipulation, cleaning and observation by the experimenter. In addition, we describe the different enrichment items, their order in the cage, the frequency of renewal and their cleaning and sterilization procedures. The total length of the protocol is 6 weeks.