Literature DB >> 20723536

Targeted messenger RNA profiling of transfected breast cancer gene in a living cell.

D Nawarathna1, R Chang, E Nelson, H Kumar Wickramasinghe.   

Abstract

Selective messenger RNA (mRNA) profiling of transfected breast cancer gene expression in a living cell is demonstrated. Atomic force microscope (AFM) probe tips are structurally modified to create a dielectrophoretic force that attracts mRNA molecules within the cell nucleus. The tip end is chemically modified to hybridize only to the target mRNA from a pool of molecules within the nucleus. We successfully combined this scheme with standard assay techniques to develop an assay technology that can be used for early disease detection and basic studies in cell biology.
Copyright © 2010 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2010        PMID： 20723536      PMCID： PMC2980590          DOI： 10.1016/j.ab.2010.08.014

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  8 in total

1.  Micropatterning of living cells on a heterogeneously wetted surface.

Authors:  Yuli Wang; Christopher E Sims; Paul Marc; Mark Bachman; G P Li; Nancy L Allbritton
Journal:  Langmuir       Date:  2006-09-12       Impact factor: 3.882

2.  Broadening cell selection criteria with micropallet arrays of adherent cells.

Authors:  Yuli Wang; Grace Young; Phillip C Aoto; Jeng-Hao Pai; Mark Bachman; G P Li; Christopher E Sims; Nancy L Allbritton
Journal:  Cytometry A       Date:  2007-10       Impact factor: 4.355

3.  Selective probing of mRNA expression levels within a living cell.

Authors:  D Nawarathna; T Turan; H Kumar Wickramasinghe
Journal:  Appl Phys Lett       Date:  2009-08-27       Impact factor: 3.791

4.  Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection.

Authors:  J J Chen; R Wu; P C Yang; J Y Huang; Y P Sher; M H Han; W C Kao; P J Lee; T F Chiu; F Chang; Y W Chu; C W Wu; K Peck
Journal:  Genomics       Date:  1998-08-01       Impact factor: 5.736

Review 5.  Exploring the new world of the genome with DNA microarrays.

Authors:  P O Brown; D Botstein
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

6.  Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells

Authors: 
Journal:  Methods       Date:  1996-12       Impact factor: 3.608

7.  Sorting and expansion of murine embryonic stem cell colonies using micropallet arrays.

Authors:  Hamed Shadpour; Christopher E Sims; Randy J Thresher; Nancy L Allbritton
Journal:  Cytometry A       Date:  2009-02       Impact factor: 4.355

8.  mRNA detection of individual cells with the single cell nanoprobe method compared with in situ hybridization.

Authors:  Hironori Uehara; Yuji Kunitomi; Atsushi Ikai; Toshiya Osada
Journal:  J Nanobiotechnology       Date:  2007-10-10       Impact factor: 10.435
  8 in total
  3 in total

1.  Nanoneedle-Based Materials for Intracellular Studies.

Authors:  Julia E Sero; Molly M Stevens
Journal:  Adv Exp Med Biol       Date:  2021       Impact factor: 2.622

2.  Compartmental genomics in living cells revealed by single-cell nanobiopsy.

Authors:  Paolo Actis; Michelle M Maalouf; Hyunsung John Kim; Akshar Lohith; Boaz Vilozny; R Adam Seger; Nader Pourmand
Journal:  ACS Nano       Date:  2013-12-04       Impact factor: 15.881

Review 3.  Nanopipettes as Monitoring Probes for the Single Living Cell: State of the Art and Future Directions in Molecular Biology.

Authors:  Gonca Bulbul; Gepoliano Chaves; Joseph Olivier; Rifat Emrah Ozel; Nader Pourmand
Journal:  Cells       Date:  2018-06-06       Impact factor: 6.600
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.