| Literature DB >> 20719272 |
Guilhem Velve-Casquillas1, Judite Costa, Frédérique Carlier-Grynkorn, Adeline Mayeux, Phong T Tran.
Abstract
Recent development in soft lithography and microfluidics enables biologists to create tools to control the cellular microenvironment. One such control is the ability to quickly change the temperature of the cells. Genetic model organism such as fission yeast has been useful for studies of the cell cytoskeleton. In particular, the dynamic microtubule cytoskeleton responds to changes in temperature. In addition, there are temperature-sensitive mutations of cytoskeletal proteins. We describe here the fabrication and use of a microfluidic device to quickly and reversibly change cellular temperature between 2 degrees C and 50 degrees C. We demonstrate the use of this device while imaging at high-resolution microtubule dynamics in fission yeast. 2010 Elsevier Inc. All rights reserved.Entities:
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Year: 2010 PMID： 20719272 PMCID： PMC2997723 DOI： 10.1016/S0091-679X(10)97011-8
Source DB: PubMed Journal: Methods Cell Biol ISSN： 0091-679X Impact factor: 1.441