| Literature DB >> 20717843 |
Dongbo Sun1, Jianfa Wang, Rui Wu, Chunren Wang, Xianjing He, Jiasan Zheng, Huanmin Yang.
Abstract
A set of four specific primers for six regions of kmt1 gene from a species specific region was designed for developing the loop-mediated isothermal amplification diagnostic method of swine Pasteurella multocida (Pm-LAMP). After the Pm-LAMP was carried out at 63°C for 1 h, the LAMP products could be visually confirmed using fluorescent dyes as detection reagent under UV-illumination. In sensitivity, the detection limit of the Pm-LAMP was 10 cfu/mL, and was 1 log less than that of the PCR method. In specificity, the Pm-LAMP did not amplify genomic DNA of swine common respiratory pathogens. Furthermore, based on results for clinical swab samples (n = 31) using PCR detection as golden standard, relative sensitivity of the Pm-LAMP was 100%, relative specificity of the Pm-LAMP was 90.9%, and percentage of observation agreement was 93.5% (Kappa = 0.85). The Pm-LAMP method should be a useful diagnostic tool for rapid and visible detection of swine Pasteurella multocida.Entities:
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Year: 2010 PMID： 20717843 DOI： 10.1007/s11259-010-9433-y
Source DB: PubMed Journal: Vet Res Commun ISSN： 0165-7380 Impact factor: 2.459