| Literature DB >> 20680839 |
Dmitry Y Guschin1, Adam J Waite, George E Katibah, Jeffrey C Miller, Michael C Holmes, Edward J Rebar.
Abstract
The development of zinc finger nucleases for targeted gene modification can benefit from rapid functional assays that directly quantify activity at the endogenous target. Here we describe a simple procedure for quantifying mutations that result from DNA double-strand break repair via non-homologous end joining. The assay is based on the ability of the Surveyor nuclease to selectively cleave distorted duplex DNA formed via cross-annealing of mutated and wild-type sequence.Mesh：
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Year: 2010 PMID： 20680839 DOI： 10.1007/978-1-60761-753-2_15
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745