Literature DB >> 20680662

Stereospecific assignment of the NH2 resonances from the primary amides of asparagine and glutamine side chains in isotopically labeled proteins.

L P McIntosh1, E Brun, L E Kay.   

Abstract

An HMQC-based pulse scheme is presented for the stereospecific assignment of asparagineand glutamine side-chain amide protons. The approach makes use of the recently developedquantitative-J correlation spectroscopy [Bax, A. et al. (1994) Methods Enzymol., 239,79-105] to distinguish the E and Z primary amide protons and, as such, eliminates theneed for assignments derived from more time-consuming and potentially ambiguous NOEmethods. An application of this method to a uniformly 15N,13C-labeled cellulose-bindingdomain is presented. When used in combination with a NOESY-HSQC experiment, thepredominant chi2 dihedral angles of two asparagine side chains in this protein can also bedefined.

Entities:  

Year:  1997        PMID： 20680662     DOI： 10.1023/A:1018635110491

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  10 in total

1.  H2NCO-E.COSY, a simple method for the sterospecific assignment of side-chain amide protons in proteins.

Authors:  F Löhr; H Rüterjans
Journal:  J Magn Reson       Date:  1997-01       Impact factor: 2.229

2.  Assignment of aliphatic side-chain 1HN/15N resonances in perdeuterated proteins.

Authors:  B T Farmer; R A Venters
Journal:  J Biomol NMR       Date:  1996-01       Impact factor: 2.835

3.  Tertiary templates for proteins. Use of packing criteria in the enumeration of allowed sequences for different structural classes.

Authors:  J W Ponder; F M Richards
Journal:  J Mol Biol       Date:  1987-02-20       Impact factor: 5.469

4.  Measurement of amide 15N-1H one-bond couplings in proteins using accordion heteronuclear-shift-correlation experiments.

Authors:  J R Tolman; J H Prestegard
Journal:  J Magn Reson B       Date:  1996-09

Review 5.  Measurement of homo- and heteronuclear J couplings from quantitative J correlation.

Authors:  A Bax; G W Vuister; S Grzesiek; F Delaglio; A C Wang; R Tschudin; G Zhu
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

6.  Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi CenC determined by nuclear magnetic resonance spectroscopy.

Authors:  P E Johnson; M D Joshi; P Tomme; D G Kilburn; L P McIntosh
Journal:  Biochemistry       Date:  1996-11-12       Impact factor: 3.162

7.  The high-resolution, three-dimensional solution structure of human interleukin-4 determined by multidimensional heteronuclear magnetic resonance spectroscopy.

Authors:  R Powers; D S Garrett; C J March; E A Frieden; A M Gronenborn; G M Clore
Journal:  Biochemistry       Date:  1993-07-06       Impact factor: 3.162

8.  Backbone 1H and 15N resonance assignments of the N-terminal SH3 domain of drk in folded and unfolded states using enhanced-sensitivity pulsed field gradient NMR techniques.

Authors:  O Zhang; L E Kay; J P Olivier; J D Forman-Kay
Journal:  J Biomol NMR       Date:  1994-11       Impact factor: 2.835

9.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors:  M Piotto; V Saudek; V Sklenár
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835

10.  1H, 13C and 15N random coil NMR chemical shifts of the common amino acids. I. Investigations of nearest-neighbor effects.

Authors:  D S Wishart; C G Bigam; A Holm; R S Hodges; B D Sykes
Journal:  J Biomol NMR       Date:  1995-01       Impact factor: 2.835
  10 in total
  9 in total

1.  Solution structure and stability of the anti-sigma factor AsiA: implications for novel functions.

Authors:  Jeffrey L Urbauer; Mario F Simeonov; Ramona J Bieber Urbauer; Karen Adelman; Joshua M Gilmore; Edward N Brody
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-05       Impact factor: 11.205

2.  Chemical shift and secondary structure conservation of the PNT/SAM domains from the ets family of transcription factors.

Authors:  Cameron D Mackereth; Manuela Schärpf; Lisa N Gentile; Lawrence P McIntosh
Journal:  J Biomol NMR       Date:  2002-09       Impact factor: 2.835

3.  Identification and structural characterization of a CBP/p300-binding domain from the ETS family transcription factor GABP alpha.

Authors:  Hyun-Seo Kang; Mary L Nelson; Cameron D Mackereth; Manuela Schärpf; Barbara J Graves; Lawrence P McIntosh
Journal:  J Mol Biol       Date:  2008-01-30       Impact factor: 5.469

4.  A Second Backbone: The Contribution of a Buried Asparagine Ladder to the Global and Local Stability of a Leucine-Rich Repeat Protein.

Authors:  Sean A Klein; Ananya Majumdar; Doug Barrick
Journal:  Biochemistry       Date:  2019-08-06       Impact factor: 3.162

5.  Stereospecific assignment of the asparagine and glutamine sidechain amide protons in proteins from chemical shift analysis.

Authors:  Tobias Harsch; Philipp Schneider; Bärbel Kieninger; Harald Donaubauer; Hans Robert Kalbitzer
Journal:  J Biomol NMR       Date:  2017-02-15       Impact factor: 2.835

6.  Solution structure and properties of AlgH from Pseudomonas aeruginosa.

Authors:  Jeffrey L Urbauer; Aaron B Cowley; Hayley P Broussard; Henry T Niedermaier; Ramona J Bieber Urbauer
Journal:  Proteins       Date:  2015-04-29

7.  Simultaneous NMR assignment of backbone and side chain amides in large proteins with IS-TROSY.

Authors:  Aizhuo Liu; Yue Li; Lishan Yao; Honggao Yan
Journal:  J Biomol NMR       Date:  2006-11-08       Impact factor: 2.835

8.  Selective (15)N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy.

Authors:  Chan Cao; Jia-Liang Chen; Yin Yang; Feng Huang; Gottfried Otting; Xun-Cheng Su
Journal:  J Biomol NMR       Date:  2014-07-08       Impact factor: 2.835

9.  TROSY of side-chain amides in large proteins.

Authors:  Aizhuo Liu; Lishan Yao; Yue Li; Honggao Yan
Journal:  J Magn Reson       Date:  2007-02-14       Impact factor: 2.229
  9 in total

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