PURPOSE: The aim of this study was to assess the effects of acyclic retinoid (ACR) and vitamin K(2) (VK(2)) in HL-60 cells. METHODS: We used HL-60 cells, and the Trypan Blue dye exclusion method was used for cell proliferation assays. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used a FITC BrdU Flow KIT and flow cytometry. Total extracted and equivalent amounts of protein were examined by Western blotting using specific antibodies. RESULTS: ACR and VK(2) dose dependently inhibited the proliferation of HL-60 cells. These two agents in combination synergistically inhibited cell growth and induced apoptosis. VK(2) inhibited activation of the Ras/MAPK signaling pathway, and ACR plus VK(2) cooperatively inhibited phosphorylation of RXRα and the growth of HL-60 cells. Moreover, ACR and VK(2) induced increases in G0/G1 phase HL-60 cells, alone and synergistically in combination. CONCLUSION: The synergistic effects of ACR and VK(2) on HL-60 cells may provide a novel strategy for treating leukemia.
PURPOSE: The aim of this study was to assess the effects of acyclic retinoid (ACR) and vitamin K(2) (VK(2)) in HL-60 cells. METHODS: We used HL-60 cells, and the Trypan Blue dye exclusion method was used for cell proliferation assays. For detection of apoptosis, the Annexin V-binding capacity of treated cells was examined by flow cytometry. To evaluate the cell cycle, we used a FITC BrdU Flow KIT and flow cytometry. Total extracted and equivalent amounts of protein were examined by Western blotting using specific antibodies. RESULTS: ACR and VK(2) dose dependently inhibited the proliferation of HL-60 cells. These two agents in combination synergistically inhibited cell growth and induced apoptosis. VK(2) inhibited activation of the Ras/MAPK signaling pathway, and ACR plus VK(2) cooperatively inhibited phosphorylation of RXRα and the growth of HL-60 cells. Moreover, ACR and VK(2) induced increases in G0/G1 phase HL-60 cells, alone and synergistically in combination. CONCLUSION: The synergistic effects of ACR and VK(2) on HL-60 cells may provide a novel strategy for treating leukemia.
Authors: R Matsushima-Nishiwaki; M Okuno; S Adachi; T Sano; K Akita; H Moriwaki; S L Friedman; S Kojima Journal: Cancer Res Date: 2001-10-15 Impact factor: 12.701
Authors: Masumi Suzui; Muneyuki Masuda; Jin T E Lim; Chris Albanese; Richard G Pestell; I Bernard Weinstein Journal: Cancer Res Date: 2002-07-15 Impact factor: 12.701