BACKGROUND: Mesenchymal stem cell transplantation is a promising new therapy to improve cardiac function after myocardial infarction (MI). The electrophysiological consequences of MSC implantation has not been systematically studied. METHODS: We investigated the electrophysiological and arrhythmogenic effects of mesenchymal stem cells (MSCs) therapy in experimental infarction model. Rats were subjected to MI operation by LAD ligation and randomly allocated to receive intramyocardially injection PBS (MI-PBS) or 5 × 10(5) EGFP labeled MSCs (MI-MSCs). Electrophysiological study, histological examination, and western blotting were performed 2 weeks after cell transplantation. RESULTS: Programmed electrical stimulation (PES) showed a significant reduced inducible ventricular tachycardias (VTs), raised ventricular fibrillation threshold (VFT) and prolonged ventricular effective refractory period (VERP) in MSC-treated rats compared to PBS-treated animals. MSC implantation led to markedly longer action potential duration (APD) and shorter activation time (AT) in infarcted border zone (IBZ) of left ventricular epicardium compared with PBS-treated hearts. Histological study revealed that fibrotic area and collagen deposition in infarcted region were significantly lower in MI-MSC group than in MI-PBS group. Abnormal alterations of Connexin 43 including reduction and lateralization were significantly attenuated by MSC treatment. CONCLUSIONS: This study provide strong evidence that MSC implantation ameliorates interstitial fibrosis and the remodeling of gap junction, attenuates focal heterogeneity of reporlarization and conduction and reduces vulnerability to VTs. The results suggest that MSC transplantation might emerge as a new preventive strategy against VAs besides improving cardiac performance in ischemic heart disease.
BACKGROUND: Mesenchymal stem cell transplantation is a promising new therapy to improve cardiac function after myocardial infarction (MI). The electrophysiological consequences of MSC implantation has not been systematically studied. METHODS: We investigated the electrophysiological and arrhythmogenic effects of mesenchymal stem cells (MSCs) therapy in experimental infarction model. Rats were subjected to MI operation by LAD ligation and randomly allocated to receive intramyocardially injection PBS (MI-PBS) or 5 × 10(5) EGFP labeled MSCs (MI-MSCs). Electrophysiological study, histological examination, and western blotting were performed 2 weeks after cell transplantation. RESULTS: Programmed electrical stimulation (PES) showed a significant reduced inducible ventricular tachycardias (VTs), raised ventricular fibrillation threshold (VFT) and prolonged ventricular effective refractory period (VERP) in MSC-treated rats compared to PBS-treated animals. MSC implantation led to markedly longer action potential duration (APD) and shorter activation time (AT) in infarcted border zone (IBZ) of left ventricular epicardium compared with PBS-treated hearts. Histological study revealed that fibrotic area and collagen deposition in infarcted region were significantly lower in MI-MSC group than in MI-PBS group. Abnormal alterations of Connexin 43 including reduction and lateralization were significantly attenuated by MSC treatment. CONCLUSIONS: This study provide strong evidence that MSC implantation ameliorates interstitial fibrosis and the remodeling of gap junction, attenuates focal heterogeneity of reporlarization and conduction and reduces vulnerability to VTs. The results suggest that MSC transplantation might emerge as a new preventive strategy against VAs besides improving cardiac performance in ischemic heart disease.
Authors: Daniel Adesse; Regina Coeli Goldenberg; Fabio S Fortes; Dumitru A Iacobas; Sanda Iacobas; Antonio Carlos Campos de Carvalho; Maria de Narareth Meirelles; Huan Huang; Milena B Soares; Herbert B Tanowitz; Luciana Ribeiro Garzoni; David C Spray Journal: Adv Parasitol Date: 2011 Impact factor: 3.870
Authors: Nikhil C Panda; Sean T Zuckerman; Olurotimi O Mesubi; David S Rosenbaum; Marc S Penn; J Kevin Donahue; Eben Alsberg; Kenneth R Laurita Journal: J Interv Card Electrophysiol Date: 2014-09-19 Impact factor: 1.900
Authors: Andrey A Karpov; Maxim V Puzanov; Dmitry Yu Ivkin; Marina V Krasnova; Nikita A Anikin; Pavel M Docshin; Olga M Moiseeva; Michael M Galagudza Journal: Int J Exp Pathol Date: 2019-04-24 Impact factor: 1.925
Authors: Joshua Mayourian; Timothy J Cashman; Delaine K Ceholski; Bryce V Johnson; David Sachs; Deepak A Kaji; Susmita Sahoo; Joshua M Hare; Roger J Hajjar; Eric A Sobie; Kevin D Costa Journal: Circ Res Date: 2017-06-22 Impact factor: 17.367
Authors: Shwetha Mureli; Christopher P Gans; Dan J Bare; David L Geenen; Nalin M Kumar; Kathrin Banach Journal: Am J Physiol Heart Circ Physiol Date: 2012-12-15 Impact factor: 4.733