BACKGROUND: Calcium channel blockers are effective antihypertensive agents, but they may affect many metabolic processes, including bone metabolism. PURPOSE: The aim of this study was to evaluate by radiographic, histologic, and biochemical analyses the effects of amlodipine on bone healing of a defect simulating a fracture in mandibular ramus of rats. METHODS: Fifty male Wistar rats were submitted to the same unilateral surgical procedure simulating a mandibular fracture. Experimental group received oral doses of amlodipine 0.04 mg/rat/day starting 12 days before procedure, while control group received water. Animals were sacrificed at 1, 7, 14, 30, and 90 days postoperatively. Numerical values were submitted to statistical analyses. RESULTS: Radiographic analysis showed larger radiolucent area into bone defect to the experimental group at the periods of 14 (p = 0.016), 30 (p = 0.009), and 90 (p = 0.028) days. In the histological analysis, the experimental group had a slight delay in the chronology of the repair process. In the histomorphometric analysis, the experimental group presented significant lowering of newly formed bone volume at 7 and 14 days periods (p = 0.049). There was a significant decrease of alkaline phosphatase levels in experimental group in the initial periods (p = 0.049). CONCLUSIONS: It was concluded that chronic use of amlodipine compromised bone neoformation in the repairing process of surgical defect in the mandibular ramus of rats, but no precluded occurrence of fracture consolidation.
BACKGROUND: Calcium channel blockers are effective antihypertensive agents, but they may affect many metabolic processes, including bone metabolism. PURPOSE: The aim of this study was to evaluate by radiographic, histologic, and biochemical analyses the effects of amlodipine on bone healing of a defect simulating a fracture in mandibular ramus of rats. METHODS: Fifty male Wistar rats were submitted to the same unilateral surgical procedure simulating a mandibular fracture. Experimental group received oral doses of amlodipine 0.04 mg/rat/day starting 12 days before procedure, while control group received water. Animals were sacrificed at 1, 7, 14, 30, and 90 days postoperatively. Numerical values were submitted to statistical analyses. RESULTS: Radiographic analysis showed larger radiolucent area into bone defect to the experimental group at the periods of 14 (p = 0.016), 30 (p = 0.009), and 90 (p = 0.028) days. In the histological analysis, the experimental group had a slight delay in the chronology of the repair process. In the histomorphometric analysis, the experimental group presented significant lowering of newly formed bone volume at 7 and 14 days periods (p = 0.049). There was a significant decrease of alkaline phosphatase levels in experimental group in the initial periods (p = 0.049). CONCLUSIONS: It was concluded that chronic use of amlodipine compromised bone neoformation in the repairing process of surgical defect in the mandibular ramus of rats, but no precluded occurrence of fracture consolidation.
Authors: Brandon A Coates; Jennifer A McKenzie; Evan G Buettmann; Xiaochen Liu; Paul M Gontarz; Bo Zhang; Matthew J Silva Journal: Bone Date: 2019-07-29 Impact factor: 4.398
Authors: Yusuf Atalay; Mehmet Fatih Bozkurt; Yucel Gonul; Omer Cakmak; Kamil Serkan Agacayak; Ibrahim Köse; Omer Hazman; Hikmet Keles; Ozan Turamanlar; Mehmet Eroglu Journal: Drug Des Devel Ther Date: 2015-04-07 Impact factor: 4.162
Authors: Marco Rohde; Josefin Ziebart; Timo Kirschstein; Tina Sellmann; Katrin Porath; Friederike Kühl; Bachir Delenda; Christian Bahls; Ursula van Rienen; Rainer Bader; Rüdiger Köhling Journal: Front Bioeng Biotechnol Date: 2019-12-12