Literature DB >> 2066385

A new method for the purification of the influenza A virus neuraminidase.

J L McKimm-Breschkin1, J B Caldwell, R E Guthrie, A A Kortt.   

Abstract

A rapid new method for the purification of neuraminidase (NA) heads from influenza A virus is described. Virus was pelleted directly from allantoic fluid and was digested with pronase. The cores were removed by centrifugation, redigested and the released NA heads were pooled and concentrated. The NA was separated from all contaminating proteins in a single step on a Superose 12 column. The purified material was suitable for both crystallography and for the production of monospecific antisera.

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Year:  1991        PMID： 2066385     DOI： 10.1016/0166-0934(91)90192-3

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  10 in total

1.  Analysis of inhibitor binding in influenza virus neuraminidase.

Authors:  B J Smith; P M Colman; M Von Itzstein; B Danylec; J N Varghese
Journal:  Protein Sci       Date:  2001-04       Impact factor: 6.725

2.  Target-induced formation of neuraminidase inhibitors from in vitro virtual combinatorial libraries.

Authors:  Matthias Hochgürtel; Heiko Kroth; Dorothea Piecha; Michael W Hofmann; Claude Nicolau; Sonja Krause; Otmar Schaaf; Gabriele Sonnenmoser; Alexey V Eliseev
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

3.  Structural evidence for a second sialic acid binding site in avian influenza virus neuraminidases.

Authors:  J N Varghese; P M Colman; A van Donkelaar; T J Blick; A Sahasrabudhe; J L McKimm-Breschkin
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-28       Impact factor: 11.205

4.  Structure of an Influenza A virus N9 neuraminidase with a tetrabrachion-domain stalk.

Authors:  Victor A Streltsov; Peter M Schmidt; Jennifer L McKimm-Breschkin
Journal:  Acta Crystallogr F Struct Biol Commun       Date:  2019-01-23       Impact factor: 1.056

5.  Binding affinity of influenza virus N9 neuraminidase with Fab fragments of monoclonal antibodies NC10 and NC41.

Authors:  L C Gruen; T L McInerney; R G Webster; D C Jackson
Journal:  J Protein Chem       Date:  1993-06

6.  Determination of Rigidity of Protein Bound Au(144) Clusters by Electron Cryomicroscopy.

Authors:  Jonathan Z Sexton; Christopher J Ackerson
Journal:  J Phys Chem C Nanomater Interfaces       Date:  2010-09-30       Impact factor: 4.126

7.  Structural and functional basis of resistance to neuraminidase inhibitors of influenza B viruses.

Authors:  Aaron J Oakley; Susan Barrett; Thomas S Peat; Janet Newman; Victor A Streltsov; Lynne Waddington; Takehiko Saito; Masato Tashiro; Jennifer L McKimm-Breschkin
Journal:  J Med Chem       Date:  2010-09-09       Impact factor: 7.446

8.  Mutations in a conserved residue in the influenza virus neuraminidase active site decreases sensitivity to Neu5Ac2en-derived inhibitors.

Authors:  J L McKimm-Breschkin; A Sahasrabudhe; T J Blick; M McDonald; P M Colman; G J Hart; R C Bethell; J N Varghese
Journal:  J Virol       Date:  1998-03       Impact factor: 5.103

9.  A generic system for the expression and purification of soluble and stable influenza neuraminidase.

Authors:  Peter M Schmidt; Rebecca M Attwood; Peter G Mohr; Susan A Barrett; Jennifer L McKimm-Breschkin
Journal:  PLoS One       Date:  2011-02-07       Impact factor: 3.240

10.  Optimisation of Neuraminidase Expression for Use in Drug Discovery by Using HEK293-6E Cells.

Authors:  Ashley C Campbell; John J Tanner; Kurt L Krause
Journal:  Viruses       Date:  2021-09-22       Impact factor: 5.048
  10 in total

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