| Literature DB >> 20659711 |
Xiaoyang Zhao1, Zhuo Lv, Lei Liu, Liu Wang, Man Tong, Qi Zhou.
Abstract
Knockout Brown Norway (BN) rat could be a useful disease model for human disorders, however, a failure to derive embryonic stem (ES) cells disturbs the further development of the model. In this study, we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley (SD) rats. The BN rat ES cells expressed the key transcription factors, and were able to form embryonic bodies (EBs) when being differentiated in vitro. After injecting the BN rat ES cells into the SD rat blastocysts, high-contribution chimeric rats were generated and could survive to their adulthood. Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future. Copyright 2010 Institute of Genetics and Developmental Biology and the Genetics Society of China. Published by Elsevier Ltd. All rights reserved.Entities:
Mesh：
Year: 2010 PMID： 20659711 DOI： 10.1016/S1673-8527(09)60066-7
Source DB: PubMed Journal: J Genet Genomics ISSN： 1673-8527 Impact factor: 4.275