Literature DB >> 2065666

Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.

Y Usuda1, A Kubota, A J Berk, H Handa.   

Abstract

One of the general transcription factors, TFIIA, was purified to homogeneity from HeLa cell nuclear extracts by yeast TFIID affinity chromatography. Human TFIIA had a molecular weight of approximately 38 kd. It was able to associate with the complex formed by yeast TFIID and the TATA elements of the adenovirus E4 and ML promoters, and the HSP70 promoter. The association extended the protected region on each TATA element by yeast TFIID from DNase I digestion. Affinity-purified TFIIA was also able to stimulate transcription from the E4 and ML promoters in in vitro reconstituted systems.

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Year:  1991        PMID： 2065666      PMCID： PMC452922          DOI： 10.1002/j.1460-2075.1991.tb07767.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  40 in total

Review 1.  RNA polymerase B (II) and general transcription factors.

Authors:  M Sawadogo; A Sentenac
Journal:  Annu Rev Biochem       Date:  1990       Impact factor: 23.643

2.  Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.

Authors:  M Sawadogo; R G Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1985-07       Impact factor: 11.205

3.  Purification and characterization of a specific RNA polymerase II transcription factor.

Authors:  M Samuels; P A Sharp
Journal:  J Biol Chem       Date:  1986-02-15       Impact factor: 5.157

4.  Interactions between RNA polymerase II, factors, and template leading to accurate transcription.

Authors:  A Fire; M Samuels; P A Sharp
Journal:  J Biol Chem       Date:  1984-02-25       Impact factor: 5.157

5.  Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.

Authors:  M Sawadogo; R G Roeder
Journal:  Cell       Date:  1985-11       Impact factor: 41.582

6.  Separation and characterization of factors mediating accurate transcription by RNA polymerase II.

Authors:  M Samuels; A Fire; P A Sharp
Journal:  J Biol Chem       Date:  1982-12-10       Impact factor: 5.157

7.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

8.  Isolation of three proteins that bind to mammalian RNA polymerase II.

Authors:  M Sopta; R W Carthew; J Greenblatt
Journal:  J Biol Chem       Date:  1985-08-25       Impact factor: 5.157

9.  Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor.

Authors:  N G Miyamoto; V Moncollin; M Wintzerith; R Hen; J M Egly; P Chambon
Journal:  Nucleic Acids Res       Date:  1984-12-11       Impact factor: 16.971

10.  Is actin a transcription initiation factor for RNA polymerase B?

Authors:  J M Egly; N G Miyamoto; V Moncollin; P Chambon
Journal:  EMBO J       Date:  1984-10       Impact factor: 11.598
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  14 in total

1.  The general transcription factors IIA, IIB, IIF, and IIE are required for RNA polymerase II transcription from the human U1 small nuclear RNA promoter.

Authors:  T C Kuhlman; H Cho; D Reinberg; N Hernandez
Journal:  Mol Cell Biol       Date:  1999-03       Impact factor: 4.272

2.  TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

Authors:  D K Lee; J DeJong; S Hashimoto; M Horikoshi; R G Roeder
Journal:  Mol Cell Biol       Date:  1992-11       Impact factor: 4.272

3.  Transcription factor IIA is inactivated during terminal differentiation of avian erythroid cells.

Authors:  J Bungert; R Waldschmidt; I Kober; K H Seifart
Journal:  Proc Natl Acad Sci U S A       Date:  1992-12-15       Impact factor: 11.205

4.  Factors involved in specific transcription by mammalian RNA polymerase II: purification and analysis of transcription factor IIA and identification of transcription factor IIJ.

Authors:  P Cortes; O Flores; D Reinberg
Journal:  Mol Cell Biol       Date:  1992-01       Impact factor: 4.272

Review 5.  Coactivators and TAFs of transcription activation in wheat.

Authors:  K B Washburn; E A Davis; S Ackerman
Journal:  Plant Mol Biol       Date:  1997-12       Impact factor: 4.076

6.  Transcription factors required for the expression of Xenopus laevis selenocysteine tRNA in vitro.

Authors:  W Meissner; I Wanandi; P Carbon; A Krol; K H Seifart
Journal:  Nucleic Acids Res       Date:  1994-02-25       Impact factor: 16.971

7.  Identification of three mammalian proteins that bind to the yeast TATA box protein TFIID.

Authors:  B Coulombe; M Killeen; P Liljelund; B Honda; H Xiao; C J Ingles; J Greenblatt
Journal:  Gene Expr       Date:  1992

8.  Mediators of activation of fushi tarazu gene transcription by BmFTZ-F1.

Authors:  F Q Li; H Ueda; S Hirose
Journal:  Mol Cell Biol       Date:  1994-05       Impact factor: 4.272

9.  Transcription factor IIA stimulates the expression of classical polIII-genes.

Authors:  W Meissner; R Holland; R Waldschmidt; K H Seifart
Journal:  Nucleic Acids Res       Date:  1993-02-25       Impact factor: 16.971

10.  Human general transcription factor TFIIA: characterization of a cDNA encoding the small subunit and requirement for basal and activated transcription.

Authors:  J DeJong; R Bernstein; R G Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1995-04-11       Impact factor: 11.205
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