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Literature DB >> 2065365

Pulsed-field gel electrophoretic analysis of Schizophyllum commune chromosomal DNA.

Abstract

Six chromosomal DNA bands of Schizophyllum commune have been resolved using transverse alternating field electrophoresis. The estimated sizes of the chromosomal DNAs ranged from 5.1 to 1.2 megabase pairs (Mb), the total genome size being approximately 35-36 Mb. Chromosomal length polymorphisms were found between the two S. commune isolates examined. The DNA bands corresponding to the two chromosomes containing the A and B mating-type loci were identified in hybridization experiments using probes specific to their respective linkage groups. The utility of eluted chromosomal DNAs as hybridization probes to select clones from genomic libraries, and the use of these clones in transformation experiments to identify genes of interest, are discussed.

Entities: Species

Mesh：

Substances：
DNA, Fungal

Year: 1991 PMID： 2065365 DOI： 10.1007/BF00326286

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

20 in total

1. Changes in mate recognition through alterations of pheromones and receptors in the multisexual mushroom fungus Schizophyllum commune.

Authors: T J Fowler; M F Mitton; L J Vaillancourt; C A Raper
Journal: Genetics Date: 2001-08 Impact factor: 4.562

2. Scooter, a new active transposon in Schizophyllum commune, has disrupted two genes regulating signal transduction.

Authors: T J Fowler; M F Mitton
Journal: Genetics Date: 2000-12 Impact factor: 4.562

Review 3. Chromosome-length polymorphism in fungi.

Authors: M E Zolan
Journal: Microbiol Rev Date: 1995-12

4. A single mating-type locus composed of homeodomain genes promotes nuclear migration and heterokaryosis in the white-rot fungus Phanerochaete chrysosporium.

Authors: Timothy Y James; Maria Lee; Linda T A van Diepen
Journal: Eukaryot Cell Date: 2010-12-03

5. An electrophoretic karyotype of the cultivated mushroom--Agaricus bisporus.

Authors: S Lodder; K Gull; D Wood
Journal: Curr Genet Date: 1993-12 Impact factor: 3.886

6. Genome analysis of imperfect fungi: electrophoretic karyotyping and characterization of the nuclear gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of Curvularia lunata.

Authors: H D Osiewacz; R Ridder
Journal: Curr Genet Date: 1991-07 Impact factor: 3.886

Pulsed-field gel electrophoretic analysis of Schizophyllum commune chromosomal DNA.

1. Cloning and comparison of A alpha mating-type alleles of the Basidiomycete Schizophyllum commune.

2. Construction of a Not I restriction map of the fission yeast Schizosaccharomyces pombe genome.

3. Low repetitive DNA content in Aspergillus nidulans.

4. A membrane-filter technique for the detection of complementary DNA.

5. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis.

6. Characterization of the genome of the basidiomycete Schizophyllum commune.

7. Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques.

8. An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis.

9. An electrophoretic karyotype for yeast.

10. Sequence analysis of the URA1 gene encoding orotidine-5'-monophosphate decarboxylase of Schizophyllum commune.

1. Changes in mate recognition through alterations of pheromones and receptors in the multisexual mushroom fungus Schizophyllum commune.

2. Scooter, a new active transposon in Schizophyllum commune, has disrupted two genes regulating signal transduction.

Review 3. Chromosome-length polymorphism in fungi.

4. A single mating-type locus composed of homeodomain genes promotes nuclear migration and heterokaryosis in the white-rot fungus Phanerochaete chrysosporium.

5. An electrophoretic karyotype of the cultivated mushroom--Agaricus bisporus.

6. Genome analysis of imperfect fungi: electrophoretic karyotyping and characterization of the nuclear gene coding for glyceraldehyde-3-phosphate dehydrogenase (gpd) of Curvularia lunata.

7. A mushroom-inducing DNA sequence isolated from the Basidiomycete, Schizophyllum commune.

8. Electrophoretic karyotypes of the elm tree pathogen Ophiostoma ulmi (sensu lato).

9. Genomic organization of a cellulase gene family in Phanerochaete chrysosporium.

10. The mushroom-inducing gene Frt1 of Schizophyllum commune encodes a putative nucleotide-binding protein.