OBJECTIVE: To compare the "take" rates of skin grafts between myeloid-selective hypoxia-inducible factor (HIF) 1alpha knockout (KO) and wild-type (WT) mice. Production of the alpha subunit of HIF-1alpha is increased in healing wounds, which stimulates expression of vascular endothelial growth factor (VEGF) to promote angiogenesis. Therefore, the take rate of skin grafts may be closely associated with the presence or absence of HIF-1alpha production in the recipient bed. DESIGN: The percentage of healthy graft areas obtained by planimetry and scores for epithelialization and granulation tissue formation obtained by histopathologic analysis were compared in 12 KO and 12 WT mice following skin grafting. RESULTS: The graft take rate was significantly impaired in the KO group (P = .009), whereas epithelialization (P = .46) or granulation (P = .41) tissue formation scores did not reveal any significant differences. CONCLUSION: Hypoxia-inducible factor 1alpha in myeloid cells may be an important molecule for revascularization of avascular tissues such as skin grafts, probably owing to its stimulating effect on angiogenesis.
OBJECTIVE: To compare the "take" rates of skin grafts between myeloid-selective hypoxia-inducible factor (HIF) 1alpha knockout (KO) and wild-type (WT) mice. Production of the alpha subunit of HIF-1alpha is increased in healing wounds, which stimulates expression of vascular endothelial growth factor (VEGF) to promote angiogenesis. Therefore, the take rate of skin grafts may be closely associated with the presence or absence of HIF-1alpha production in the recipient bed. DESIGN: The percentage of healthy graft areas obtained by planimetry and scores for epithelialization and granulation tissue formation obtained by histopathologic analysis were compared in 12 KO and 12 WT mice following skin grafting. RESULTS: The graft take rate was significantly impaired in the KO group (P = .009), whereas epithelialization (P = .46) or granulation (P = .41) tissue formation scores did not reveal any significant differences. CONCLUSION:Hypoxia-inducible factor 1alpha in myeloid cells may be an important molecule for revascularization of avascular tissues such as skin grafts, probably owing to its stimulating effect on angiogenesis.
Authors: Harriet W Hopf; Jeffrey J Gibson; Adam P Angeles; James S Constant; John J Feng; Mark D Rollins; M Zamirul Hussain; Thomas K Hunt Journal: Wound Repair Regen Date: 2005 Nov-Dec Impact factor: 3.617
Authors: P J Taub; J D Marmur; W X Zhang; D Senderoff; M L Urken; L Silver; H Weinberg Journal: J Reconstr Microsurg Date: 1998-08 Impact factor: 2.873
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Authors: P J Taub; J D Marmur; W X Zhang; D Senderoff; P D Nhat; R Phelps; M L Urken; L Silver; H Weinberg Journal: Plast Reconstr Surg Date: 1998-11 Impact factor: 4.730
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Authors: Hyeran Helen Jeon; Quan Yu; Yongjian Lu; Evelyn Spencer; Chanyi Lu; Tatyana Milovanova; Yang Yang; Chenying Zhang; Olga Stepanchenko; Rameen P Vafa; Paulo G Coelho; Dana T Graves Journal: J Pathol Date: 2018-04-20 Impact factor: 7.996