Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Single-allele analysis of transcription kinetics in living mammalian cells.

Literature DB >> 20639867

Single-allele analysis of transcription kinetics in living mammalian cells.

Sharon Yunger¹, Liat Rosenfeld, Yuval Garini, Yaron Shav-Tal.

Abstract

We generated a system for in vivo visualization and analysis of mammalian mRNA transcriptional kinetics of single alleles in real time, using single-gene integrations. We obtained high-resolution transcription measurements of a single cyclin D1 allele under endogenous or viral promoter control, including quantification of temporal kinetics of transcriptional bursting, promoter firing, nascent mRNA numbers and transcription rates during the cell cycle, and in relation to DNA replication.

Entities: Gene Species

Mesh：

Substances：
Cyclin D1

Year: 2010 PMID： 20639867 DOI： 10.1038/nmeth.1482

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

21 in total

1. The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.

Authors: L D Mesner; J L Hamlin; P A Dijkwel
Journal: Proc Natl Acad Sci U S A Date: 2003-03-10 Impact factor: 11.205

2. DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters.

Authors: Anje Sporbert; Anja Gahl; Richard Ankerhold; Heinrich Leonhardt; M Cristina Cardoso
Journal: Mol Cell Date: 2002-12 Impact factor: 17.970

3. Heritable gene silencing in lymphocytes delays chromatid resolution without affecting the timing of DNA replication.

Authors: Véronique Azuara; Karen E Brown; Ruth R E Williams; Natasha Webb; Niall Dillon; Richard Festenstein; Veronica Buckle; Matthias Merkenschlager; Amanda G Fisher
Journal: Nat Cell Biol Date: 2003-07 Impact factor: 28.824

4. Mechanism of mRNA transport in the nucleus.

Authors: Diana Y Vargas; Arjun Raj; Salvatore A E Marras; Fred Russell Kramer; Sanjay Tyagi
Journal: Proc Natl Acad Sci U S A Date: 2005-11-11 Impact factor: 11.205

5. Transient anomalous diffusion of telomeres in the nucleus of mammalian cells.

Authors: I Bronstein; Y Israel; E Kepten; S Mai; Y Shav-Tal; E Barkai; Y Garini
Journal: Phys Rev Lett Date: 2009-07-02 Impact factor: 9.161

6. Visualization of single RNA transcripts in situ.

Authors: A M Femino; F S Fay; K Fogarty; R H Singer
Journal: Science Date: 1998-04-24 Impact factor: 47.728

Review 7. Single-particle tracking: applications to membrane dynamics.

Authors: M J Saxton; K Jacobson
Journal: Annu Rev Biophys Biomol Struct Date: 1997

8. RNP particles at splice junction sequences on Drosophila chorion transcripts.

Authors: Y N Osheim; O L Miller; A L Beyer
Journal: Cell Date: 1985-11 Impact factor: 41.582

9. Transcriptional pulsing of a developmental gene.

Authors: Jonathan R Chubb; Tatjana Trcek; Shailesh M Shenoy; Robert H Singer
Journal: Curr Biol Date: 2006-05-23 Impact factor: 10.834

10. The transcriptional cycle of HIV-1 in real-time and live cells.

Authors: Stéphanie Boireau; Paolo Maiuri; Eugenia Basyuk; Manuel de la Mata; Anna Knezevich; Bérangère Pradet-Balade; Volker Bäcker; Alberto Kornblihtt; Alessandro Marcello; Edouard Bertrand
Journal: J Cell Biol Date: 2007-10-22 Impact factor: 10.539

79 in total

1. Pol II caught speeding by single gene imaging.

Authors: Danielle Cannon; Jonathan R Chubb
Journal: EMBO Rep Date: 2011-12-01 Impact factor: 8.807

Review 2. Transcription goes digital.

Authors: Timothée Lionnet; Robert H Singer
Journal: EMBO Rep Date: 2012-04-02 Impact factor: 8.807

Single-allele analysis of transcription kinetics in living mammalian cells.

1. The matrix attachment region in the Chinese hamster dihydrofolate reductase origin of replication may be required for local chromatid separation.

2. DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters.

3. Heritable gene silencing in lymphocytes delays chromatid resolution without affecting the timing of DNA replication.

4. Mechanism of mRNA transport in the nucleus.

5. Transient anomalous diffusion of telomeres in the nucleus of mammalian cells.

6. Visualization of single RNA transcripts in situ.

Review 7. Single-particle tracking: applications to membrane dynamics.

8. RNP particles at splice junction sequences on Drosophila chorion transcripts.

9. Transcriptional pulsing of a developmental gene.

10. The transcriptional cycle of HIV-1 in real-time and live cells.

1. Pol II caught speeding by single gene imaging.

Review 2. Transcription goes digital.

Review 3. Random monoallelic expression of autosomal genes: stochastic transcription and allele-level regulation.

4. Design (and) principles of nuclear dynamics in Stockholm.

Review 5. Zooming in on single active genes in living mammalian cells.

6. Fast transcription rates of RNA polymerase II in human cells.

7. On the right track: following the nucleo-cytoplasmic path of an mRNA.

8. Spliceosome assembly is coupled to RNA polymerase II dynamics at the 3' end of human genes.

9. Stochastic Kinetics of Nascent RNA.

10. Transcriptional burst frequency and burst size are equally modulated across the human genome.