Literature DB >> 20638357

Optimization of a nonradioactive immunosorbent assay for p38alpha mitogen-activated protein kinase activity.

Márcia Goettert1, Ralph Graeser, Stefan A Laufer.   

Abstract

Here we describe an optimization of a nonradioactive immunosorbent p38alpha mitogen-activated protein kinase (MAPK) activity assay to determine inhibitory potency of small molecule inhibitors. The assay omits the secondary antibody and, therefore, is shorter, more accurate, and easier to handle (total assay time of 3 h). This direct assay uses a new monoclonal anti-phospho-ATF-2 (Thr69/71) peroxidase-conjugated antibody, increasing specificity and eliminating problems with cross-reactivities of secondary antibodies. Copyright 2010 Elsevier Inc. All rights reserved.

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Year:  2010        PMID： 20638357     DOI： 10.1016/j.ab.2010.07.007

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Pyridinylimidazoles as GSK3β Inhibitors: The Impact of Tautomerism on Compound Activity via Water Networks.

Authors:  Fabian Heider; Tatu Pantsar; Mark Kudolo; Francesco Ansideri; Angela De Simone; Letizia Pruccoli; Taiane Schneider; Marcia Inês Goettert; Andrea Tarozzi; Vincenza Andrisano; Stefan A Laufer; Pierre Koch
Journal:  ACS Med Chem Lett       Date:  2019-08-26       Impact factor: 4.345

2.  Discovery of novel orally active anti-inflammatory N-phenylpyrazolyl-N-glycinyl-hydrazone derivatives that inhibit TNF-α production.

Authors:  Renata B Lacerda; Leandro L da Silva; Cleverton K F de Lima; Eduardo Miguez; Ana Luisa P Miranda; Stefan A Laufer; Eliezer J Barreiro; Carlos A M Fraga
Journal:  PLoS One       Date:  2012-10-08       Impact factor: 3.240

3.  Novel potent imidazo[1,2-a]pyridine-N-Glycinyl-hydrazone inhibitors of TNF-α production: in vitro and in vivo studies.

Authors:  Renata B Lacerda; Natália M Sales; Leandro L da Silva; Roberta Tesch; Ana Luisa P Miranda; Eliezer J Barreiro; Patricia D Fernandes; Carlos A M Fraga
Journal:  PLoS One       Date:  2014-03-14       Impact factor: 3.240

4.  From 2-Alkylsulfanylimidazoles to 2-Alkylimidazoles: An Approach towards Metabolically More Stable p38α MAP Kinase Inhibitors.

Authors:  Fabian Heider; Urs Haun; Eva Döring; Mark Kudolo; Catharina Sessler; Wolfgang Albrecht; Stefan Laufer; Pierre Koch
Journal:  Molecules       Date:  2017-10-14       Impact factor: 4.411

5.  A Diverse and Versatile Regiospecific Synthesis of Tetrasubstituted Alkylsulfanylimidazoles as p38α Mitogen-Activated Protein Kinase Inhibitors.

Authors:  Francesco Ansideri; Stanislav Andreev; Annette Kuhn; Wolfgang Albrecht; Stefan A Laufer; Pierre Koch
Journal:  Molecules       Date:  2018-01-20       Impact factor: 4.411

6.  A p38 substrate-specific MK2-EGFP translocation assay for identification and validation of new p38 inhibitors in living cells: a comprising alternative for acquisition of cellular p38 inhibition data.

Authors:  Roman Anton; Silke M Bauer; Peter R W E F Keck; Stefan Laufer; Ulrich Rothbauer
Journal:  PLoS One       Date:  2014-04-17       Impact factor: 3.240

7.  Structural Optimization of a Pyridinylimidazole Scaffold: Shifting the Selectivity from p38α Mitogen-Activated Protein Kinase to c-Jun N-Terminal Kinase 3.

Authors:  Francesco Ansideri; Joana T Macedo; Michael Eitel; Ahmed El-Gokha; Dhafer S Zinad; Camilla Scarpellini; Mark Kudolo; Dieter Schollmeyer; Frank M Boeckler; Bärbel S Blaum; Stefan A Laufer; Pierre Koch
Journal:  ACS Omega       Date:  2018-07-12
  7 in total

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